PMID- 8305475
OWN - NLM
STAT- MEDLINE
DCOM- 19940315
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1204
IP  - 1
DP  - 1994 Jan 11
TI  - Dopachrome tautomerase decreases the binding of indolic melanogenesis 
      intermediates to proteins.
PG  - 53-60
AB  - Dopachrome tautomerase (DCT) is a recently characterized enzyme contributing to 
      the control of melanogenesis in mammals. The enzyme catalyzes the rearrangement 
      of L-Dopachrome (L-DC) to 5,6-dihydroxyindole 2-carboxylic acid (DHICA), while 
      the spontaneous rearrangement of L-DC leads to 5,6-dihydroxyindole (DHI). Due to 
      the lower reactivity of DHICA in comparison to DHI, DCT could provide a 
      protective mechanism against the cytotoxicity of decarboxylated indolic 
      melanogenic intermediates by limiting the formation of these highly reactive 
      decarboxylated species within melanocytes. We have followed the binding of 
      radioactive melanogenic precursors to a model protein, bovine serum albumin 
      (BSA). Using L-DC as initial melanin precursor, this binding was decreased by DCT 
      in a concentration-dependent manner. In the presence of tyrosinase, the binding 
      of L-Dopa-derived intermediates to BSA was also decreased by DCT and the 
      percentage of decrease was even higher than using L-DC as initial melanin 
      precursor. SDS-PAGE followed by fluorographic detection of radioactive bands 
      showed the formation of covalent adducts between BSA and melanin precursors, as 
      well as of aggregated forms of this protein. This aggregation was also diminished 
      by DCT. These data indicate that DCT could play a protective role against the 
      cytotoxic action of decarboxylated indoles within mammalian melanocytes.
FAU - Salinas, C
AU  - Salinas C
AD  - Department of Biochemistry and Molecular Biology, School of Medicine, University 
      of Murcia, Spain.
FAU - Garcia-Borron, J C
AU  - Garcia-Borron JC
FAU - Solano, F
AU  - Solano F
FAU - Lozano, J A
AU  - Lozano JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 0 (Protein Precursors)
RN  - 0 (Quinones)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - EC 5.- (Isomerases)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
SB  - IM
MH  - Animals
MH  - Indoles/*metabolism
MH  - *Intramolecular Oxidoreductases
MH  - Isomerases/*metabolism
MH  - Melanins/*biosynthesis
MH  - Melanocytes/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Precursors/*metabolism
MH  - Quinones/metabolism
MH  - Serum Albumin, Bovine/*metabolism
EDAT- 1994/01/11 00:00
MHDA- 1994/01/11 00:01
CRDT- 1994/01/11 00:00
PHST- 1994/01/11 00:00 [pubmed]
PHST- 1994/01/11 00:01 [medline]
PHST- 1994/01/11 00:00 [entrez]
AID - 0167-4838(94)90032-9 [pii]
AID - 10.1016/0167-4838(94)90032-9 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Jan 11;1204(1):53-60. doi: 
      10.1016/0167-4838(94)90032-9.