PMID- 8305483
OWN - NLM
STAT- MEDLINE
DCOM- 19940315
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1210
IP  - 3
DP  - 1994 Jan 20
TI  - Arachidonate 15-lipoxygenase in human corneal epithelium and 12- and 
      15-lipoxygenases in bovine corneal epithelium: comparison with other bovine 
      12-lipoxygenases.
PG  - 288-96
AB  - Lipoxygenases of bovine and human corneal epithelia were investigated. The bovine 
      epithelium contained an arachidonate 12-lipoxygenase and a 15-lipoxygenase. The 
      12-lipoxygenase was found in the microsomal fraction, while the 15-lipoxygenase 
      was mainly present in the cytosol (100,000 x g supernatant). 
      12S-Hydroxyeicosatetraenoic acid (12S-HETE) and 15S-hydroxyeicosatetraenoic acid 
      (15S-HETE) were identified by GC-MS and chiral HPLC. BW A4C, an acetohydroxamic 
      acid lipoxygenase inhibitor, reduced the biosynthesis of 12S-HETE and 15S-HETE by 
      over 90% at 10 microM. IC50 for the 12-lipoxygenase was 0.3 microM. The bovine 
      corneal 12-lipoxygenase was compared with the 12-lipoxygenases of bovine 
      platelets and leukocytes. All three enzymes metabolized 14C-labelled linoleic 
      acid and alpha-linolenic acid poorly (5-16%) in comparison with [14C]arachidonic 
      acid. [14C]Docosahexaenoic acid and [14C]4,7,10,13,16-docosapentaenoic acid 
      appeared to be less efficiently converted by the corneal enzyme than by the 
      platelet and leukocyte enzymes. Immunohistochemical analysis of the bovine 
      corneal epithelium using a polyclonal antibody against porcine leukocyte 
      12-lipoxygenase gave positive staining. The cytosol of human corneal epithelium 
      converted [14C]arachidonic acid to one prominent metabolite. The product 
      co-chromatographed with 15S-HETE on reverse phase HPLC, straight phase HPLC and 
      chiral HPLC. Our results suggest that human corneal epithelium contains a 
      15-lipoxygenase and that bovine corneal epithelium contains both a 
      15-lipoxygenase and a 12-lipoxygenase. The corneal 12-lipoxygenase appears to 
      differ catalytically from earlier described bovine 12-lipoxygenases.
FAU - Liminga, M
AU  - Liminga M
AD  - Department of Pharmaceutical Biosciences, Uppsala University Biomedical Centre, 
      Sweden.
FAU - Hornsten, L
AU  - Hornsten L
FAU - Sprecher, H W
AU  - Sprecher HW
FAU - Oliw, E H
AU  - Oliw EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 59985-28-3 (12-Hydroxy-5,8,10,14-eicosatetraenoic Acid)
RN  - 73945-47-8 (15-hydroxy-5,8,11,13-eicosatetraenoic acid)
RN  - EC 1.13.11.31 (Arachidonate 12-Lipoxygenase)
RN  - EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
SB  - IM
MH  - 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
MH  - Animals
MH  - Arachidonate 12-Lipoxygenase/*analysis
MH  - Arachidonate 15-Lipoxygenase/*analysis
MH  - Arachidonic Acid/metabolism
MH  - Cattle
MH  - Chromatography, High Pressure Liquid
MH  - Cornea/*enzymology
MH  - Epithelium/enzymology
MH  - Humans
MH  - Hydroxyeicosatetraenoic Acids/biosynthesis
MH  - Immunohistochemistry
MH  - Subcellular Fractions/enzymology
EDAT- 1994/01/20 00:00
MHDA- 1994/01/20 00:01
CRDT- 1994/01/20 00:00
PHST- 1994/01/20 00:00 [pubmed]
PHST- 1994/01/20 00:01 [medline]
PHST- 1994/01/20 00:00 [entrez]
AID - 0005-2760(94)90231-3 [pii]
AID - 10.1016/0005-2760(94)90231-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Jan 20;1210(3):288-96. doi: 
      10.1016/0005-2760(94)90231-3.