PMID- 8306317
OWN - NLM
STAT- MEDLINE
DCOM- 19940314
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 54
IP  - 3
DP  - 1994 Feb 1
TI  - Loss of heterozygosity on chromosome 13 is common only in the biologically more 
      aggressive subtypes of ovarian epithelial tumors and is associated with normal 
      retinoblastoma gene expression.
PG  - 605-9
AB  - We examined the frequencies of loss of heterozygosity on chromosome 13 in 77 
      primary ovarian epithelial tumors subdivided into cystadenomas, tumors of low 
      malignant potential, low grade carcinomas, and high grade carcinomas. Such losses 
      were found in approximately 50% of high grade carcinomas but were not detected in 
      any of the other tumor subtypes (P < 0.0001), suggesting a strong association 
      between these abnormalities and the high grade carcinoma phenotype. The tumors 
      were also examined for abnormalities in expression of the retinoblastoma 
      susceptibility gene (RB). This was assessed by immunohistochemical staining of 
      archival tumor sections with a polyclonal antibody directed against both the 
      phosphorylated and the underphosphorylated forms of the RB protein. Most of the 
      tumors, including those with allelic deletions on chromosome 13, showed normal RB 
      nuclear protein staining patterns. We conclude that loss of RB expression is not 
      a frequent event in primary ovarian carcinomas and that this gene is probably not 
      the target of the frequent allelic deletions on chromosome 13 found in high grade 
      ovarian carcinomas.
FAU - Kim, T M
AU  - Kim TM
AD  - Department of Pathology, Kenneth Norris Jr. Comprehensive Cancer Center, USC 
      School of Medicine, Los Angeles 90033.
FAU - Benedict, W F
AU  - Benedict WF
FAU - Xu, H J
AU  - Xu HJ
FAU - Hu, S X
AU  - Hu SX
FAU - Gosewehr, J
AU  - Gosewehr J
FAU - Velicescu, M
AU  - Velicescu M
FAU - Yin, E
AU  - Yin E
FAU - Zheng, J
AU  - Zheng J
FAU - D'Ablaing, G
AU  - D'Ablaing G
FAU - Dubeau, L
AU  - Dubeau L
LA  - eng
GR  - R01 CA54672/CA/NCI NIH HHS/United States
GR  - R01 CA60443/CA/NCI NIH HHS/United States
GR  - R29 CA51167/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Retinoblastoma Protein)
SB  - IM
GS  - RB
MH  - Alleles
MH  - Base Sequence
MH  - Carcinoma/genetics
MH  - Chromosomes, Human, Pair 13/*physiology
MH  - Cystadenoma/genetics
MH  - Epithelium/pathology
MH  - Female
MH  - *Gene Deletion
MH  - Gene Expression/*genetics
MH  - Genes, Retinoblastoma/*genetics
MH  - *Heterozygote
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Ovarian Neoplasms/chemistry/*genetics/*pathology
MH  - Retinoblastoma Protein/analysis/genetics
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1994 Feb 1;54(3):605-9.