PMID- 8311928
OWN - NLM
STAT- MEDLINE
DCOM- 19940324
LR  - 20190920
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 30
IP  - 2-3
DP  - 1993 Sep-Oct
TI  - Detection of fetal trisomies 21 and 18 from maternal blood using triple gradient 
      and magnetic cell sorting.
PG  - 194-201
AB  - PROBLEM: The need for an inexpensive and reproducible technique for noninvasive 
      prenatal diagnosis by fetal cell isolation from maternal blood. METHOD: For 
      enrichment of fetal cells we used a combination of triple density gradient and 
      magnetic sorting (MACS) of (anti-CD71) transferrin receptor antibody labeled 
      cells followed by fluorescence in situ hybridization (FISH) with 
      chromosome-specific DNA probes for detection of fetal aneuploidies. We identified 
      15 cases of fetal trisomy (five cases with a trisomy 18 and ten cases with a 
      trisomy 21) with subsequent chromosome-specific FISH. RESULTS: We found in all of 
      the aneuploid pregnancies that the percentage of cells with three hybridization 
      signals did not overlap with those of normal controls independent from 
      gestational ages and previous invasive procedures. CONCLUSIONS: Our new approach 
      for noninvasive prenatal diagnosis has proven to be reliable in this first 
      series.
FAU - Ganshirt-Ahlert, D
AU  - Ganshirt-Ahlert D
AD  - Department of Obstetrics and Gynecology, University of Munster, Germany.
FAU - Borjesson-Stoll, R
AU  - Borjesson-Stoll R
FAU - Burschyk, M
AU  - Burschyk M
FAU - Dohr, A
AU  - Dohr A
FAU - Garritsen, H S
AU  - Garritsen HS
FAU - Helmer, E
AU  - Helmer E
FAU - Miny, P
AU  - Miny P
FAU - Velasco, M
AU  - Velasco M
FAU - Walde, C
AU  - Walde C
FAU - Patterson, D
AU  - Patterson D
AU  - et al.
LA  - eng
GR  - NICHD HD17449/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
SB  - IM
MH  - Blood Cells/*chemistry
MH  - Cell Separation/methods
MH  - Cells, Cultured
MH  - *Chromosomes, Human, Pair 18
MH  - Down Syndrome/*diagnosis
MH  - Female
MH  - Fetal Diseases/diagnosis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Trisomy/*diagnosis
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1111/j.1600-0897.1993.tb00620.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1993 Sep-Oct;30(2-3):194-201. doi: 
      10.1111/j.1600-0897.1993.tb00620.x.