PMID- 8312455
OWN - NLM
STAT- MEDLINE
DCOM- 19940324
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 50
IP  - 1
DP  - 1994 Jan
TI  - Rat ovary produces cytokines during ovulation.
PG  - 88-94
AB  - To examine the production of cytokines by the ovary during ovulation, ovaries 
      were obtained from immature rats and from eCG/hCG-primed immature rats at 
      different stages of the ovulatory process (before hCG injection, 10 h after hCG, 
      and 20 h after hCG) and were perfused in vitro for 5 h. Large quantities of 
      interleukin (IL)-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) 
      bioactivity and smaller amounts of tumor necrosis factor-alpha (TNF alpha) and 
      IL-1 bioactivity were found in the perfusate. IL-2 and IL-3 were not detectable 
      in the perfusion media. The GM-CSF content was significantly higher in the 
      perfusate of ovulating ovaries (obtained 10 h after hCG) compared to the earlier 
      stages. Studies on preovulatory ovaries (prior to hCG injection) revealed that 
      GM-CSF release was not influenced by LH, but was markedly increased when 
      recombinant human IL-1 beta (4 ng/ml) was added to the perfusion medium. IL-6 was 
      released in similar amounts from ovaries at all stages. The identity of bioactive 
      GM-CSF was confirmed by neutralization with a specific polyclonal antibody 
      against murine GM-CSF. Size-exclusion chromatography of perfusion medium revealed 
      peaks of GM-CSF and IL-6 bioactivity at approximate molecular masses of 21-23 kDa 
      and 24-25 kDa, respectively. This study demonstrates that the rat ovary produces 
      IL-6, GM-CSF, TNF alpha, and IL-1 prior to and during the ovulatory process and 
      that there are temporal fluctuations in GM-CSF release with a peak in output at 
      ovulation.
FAU - Brannstrom, M
AU  - Brannstrom M
AD  - Department of Obstetrics and Gynaecology, University of Adelaide, Queen Elizabeth 
      Hospital, Woodville, Australia.
FAU - Norman, R J
AU  - Norman RJ
FAU - Seamark, R F
AU  - Seamark RF
FAU - Robertson, S A
AU  - Robertson SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Antibodies)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Chorionic Gonadotropin/pharmacology
MH  - Cytokines/*biosynthesis
MH  - Estradiol/metabolism
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating 
      Factor/biosynthesis/chemistry/immunology
MH  - Interleukin-6/biosynthesis/chemistry/immunology
MH  - Molecular Weight
MH  - Ovary/*metabolism
MH  - Ovulation/*physiology
MH  - Progesterone/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1095/biolreprod50.1.88 [doi]
PST - ppublish
SO  - Biol Reprod. 1994 Jan;50(1):88-94. doi: 10.1095/biolreprod50.1.88.