PMID- 8312571
OWN - NLM
STAT- MEDLINE
DCOM- 19940324
LR  - 20091119
IS  - 0340-4684 (Print)
IS  - 0340-4684 (Linking)
VI  - 19
IP  - 2
DP  - 1993
TI  - Mechanical models of pseudopod formation.
PG  - 389-97; discussion 398-9
AB  - The active locomotion of polymorphonuclear leukocytes into a glass pipette has 
      been recently reported using formyl-methionyl-leucyl-phenylalanine (fMLP) as a 
      chemoattractant. The frontal portion of the leukocyte appears clear and free of 
      granules as observed in pseudopod formation. Three possible mechanisms for 
      pseudopod formation are considered: (1) pressure-flow generated by actin-myosin 
      contraction at the rear of the cell or at the base of the pseudopod; (2) osmotic 
      pressure generated at the cell membrane, interior to the cell; and (3) actin 
      polymerization of the cell membrane at the leading edge of the pseudopod. 
      Experimental data on the movement of F-actin toward the rear of the cell, away 
      from the front, favors polymerization of G-actin to F-actin at the leading edge. 
      The active role of osmotic pressure and contraction at the base of the pseudopod 
      are possible but not yet proven.
FAU - Skalak, R
AU  - Skalak R
AD  - Institute of Biomedical Engineering, University of California-San Diego, La Jolla 
      92093-0412.
FAU - Skierczynski, B A
AU  - Skierczynski BA
FAU - Wung, S L
AU  - Wung SL
FAU - Chien, S
AU  - Chien S
FAU - Usami, S
AU  - Usami S
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Blood Cells
JT  - Blood cells
JID - 7513567
RN  - 0 (Actins)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Actins/metabolism/physiology
MH  - Animals
MH  - Cell Membrane/physiology
MH  - Cell Movement/*physiology
MH  - *Chemotaxis, Leukocyte
MH  - Humans
MH  - Mathematics
MH  - *Models, Biological
MH  - Myosins/metabolism/physiology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*physiology
RF  - 20
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
PST - ppublish
SO  - Blood Cells. 1993;19(2):389-97; discussion 398-9.