PMID- 8313987
OWN - NLM
STAT- MEDLINE
DCOM- 19940318
LR  - 20071114
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 161
IP  - 2
DP  - 1994 Feb
TI  - Leukemia inhibitory factor and neurotrophins support overlapping populations of 
      rat nodose sensory neurons in culture.
PG  - 338-44
AB  - Recent studies in this and other laboratories demonstrated that leukemia 
      inhibitory factor (LIF) can regulate differentiation and survival of nerve growth 
      factor (NGF)-dependent primary sensory neurons. To determine whether development 
      of NGF-independent sensory neurons could be similarly regulated, the present 
      study examined the effects of LIF and the related cytokine-like growth factor, 
      ciliary neurotrophic factor (CNTF), on survival of rat nodose ganglion (NG) cells 
      in culture. In addition, survival in LIF-treated cultures was compared in the 
      presence and absence of the neurotrophins brain-derived neurotrophic factor 
      (BDNF), NT-3, and NT-4, factors previously shown to target NG sensory neurons, to 
      determine whether the cytokine-like factors and neurotrophins act on the same 
      population of ganglion cells. Treatment of dissociate cultures of Embryonic Day 
      (E) 16.5 NG with LIF or CNTF (10 ng/ml) resulted in a four- to fivefold increase 
      in neuronal survival; the number of neurons supported by either factor 
      represented approximately 50% of the total NG population. In contrast, less than 
      10% of newborn NG neurons survived in the presence of LIF or CNTF, suggesting a 
      loss in responsiveness to these factors during fetal development in vivo. In 
      cultures of E16.5 ganglia, BDNF, NT-3, or NT-4 alone each increased cell survival 
      to varying degrees (BDNF > NT-4 = LIF > NT-3); NGF, on the other hand, had no 
      effect. Combining LIF with BDNF, NT-3, or NT-4 had only partially additive 
      effects on NG neuron number (LIF + BDNF, 36% greater than BDNF alone; LIF + NT-3, 
      21% greater than LIF alone; LIF + NT-4, 49% greater than NT-4 or LIF alone; P < 
      0.05), indicating that LIF and these neurotrophins support survival of 
      overlapping subsets of NG neurons in culture. To begin defining whether NG 
      neurons were capable of responding to CNTF and LIF in vivo, Northern blots were 
      used to examine expression of mRNAs coding for CNTF and LIF receptor components 
      (CNTFR alpha, gp130, and LIFR beta) in the intact ganglion. mRNAs for all three 
      receptor components were present in both E16.5 and newborn ganglia, suggesting 
      that LIF and CNTF receptors are expressed in the NG in vivo. Taken together, 
      these data demonstrate that LIF and CNTF can support survival of NGF-independent 
      sensory neurons in culture and indicate that cytokine-like growth factors may 
      play a role in regulating visceral sensory development in vivo.
FAU - Thaler, C D
AU  - Thaler CD
AD  - Department of Neurosciences, Case Western Reserve University School of Medicine, 
      Cleveland, Ohio 44106.
FAU - Suhr, L
AU  - Suhr L
FAU - Ip, N
AU  - Ip N
FAU - Katz, D M
AU  - Katz DM
LA  - eng
GR  - HL-42131/HL/NHLBI NIH HHS/United States
GR  - T32NS07118/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Interleukin-6)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Leukemia Inhibitory Factor Receptor alpha Subunit)
RN  - 0 (Lifr protein, rat)
RN  - 0 (Lymphokines)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, OSM-LIF)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Ciliary Neurotrophic Factor
MH  - Growth Inhibitors/*pharmacology
MH  - *Interleukin-6
MH  - Leukemia Inhibitory Factor
MH  - Leukemia Inhibitory Factor Receptor alpha Subunit
MH  - Lymphokines/*pharmacology
MH  - Nerve Growth Factors/pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons, Afferent/drug effects/*physiology
MH  - Nodose Ganglion/*drug effects
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptors, Cytokine/genetics
MH  - Receptors, Growth Factor/genetics
MH  - Receptors, OSM-LIF
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - S0012-1606(84)71035-9 [pii]
AID - 10.1006/dbio.1994.1035 [doi]
PST - ppublish
SO  - Dev Biol. 1994 Feb;161(2):338-44. doi: 10.1006/dbio.1994.1035.