PMID- 8314281
OWN - NLM
STAT- MEDLINE
DCOM- 19940324
LR  - 20180822
IS  - 0818-9641 (Print)
IS  - 0818-9641 (Linking)
VI  - 71 ( Pt 6)
DP  - 1993 Dec
TI  - Effect of cationization on anti-hapten antibody responses in sheep and mice.
PG  - 517-25
AB  - The effect on anti-hapten immune responses of altering the isoelectric point of 
      hapten-carrier conjugates by cationization was examined in sheep and mice. No 
      adjuvants were employed in these studies. In sheep the cationization of 
      ovalbumin-dinitrophenyl (OVA-DNP) resulted in an enhanced primary 
      anti-dinitrophenyl (DNP) response. The secondary anti-DNP responses were not 
      consistently greater for animals primed with cationized (CAT) OVA-DNP than in 
      animals primed with OVA-DNP. Similar results were obtained with mice immunized 
      with bovine serum albumin (BSA)-DNP or CAT BSA-DNP. Mice and sheep primed with 
      OVA-DNP failed to mount an anamnestic response following secondary immunization 
      with CAT OVA-DNP. Failure of a cationized hapten-carrier to stimulate a secondary 
      anti-DNP response following priming with uncationized hapten-carrier was also 
      observed in mice using BSA-DNP as the model antigen.
FAU - Altmann, K G
AU  - Altmann KG
AD  - CSIRO, Division of Animal Health, Armidale, NSW, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9006-59-1 (Ovalbumin)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - Animals
MH  - *Antibody Formation
MH  - Dinitrofluorobenzene/analogs & derivatives/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Haptens/*immunology
MH  - *Immunization
MH  - Immunization Schedule
MH  - Immunoglobulin Isotypes/analysis
MH  - Isoelectric Point
MH  - Mice
MH  - Ovalbumin/analogs & derivatives/*immunology
MH  - Serum Albumin, Bovine/*immunology
MH  - Sheep
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1038/icb.1993.57 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 1993 Dec;71 ( Pt 6):517-25. doi: 10.1038/icb.1993.57.