PMID- 8314554
OWN - NLM
STAT- MEDLINE
DCOM- 19930728
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 91
IP  - 5
DP  - 1993 Jun
TI  - Fetal nucleated cells in maternal peripheral blood: frequency and relationship to 
      gestational age.
PG  - 427-32
AB  - To determine the frequency of fetal nucleated cells in maternal peripheral blood 
      during different stages of pregnancy, 50 primigravidas were investigated by 
      determining the frequency of cells with the Y chromosome using fluorescence in 
      situ hybridization (FISH) of Y-specific repetitive sequences of the DYZ1 family. 
      Polymerase chain reaction (PCR) amplifying the same part of the DYZ1 used as the 
      probe in FISH and a single-copy Y-specific fragment was also carried out for 
      genomic DNA from the same samples. Cells with the hybridization signal were 
      detected by FISH at and after 15 weeks of pregnancy in all pregnant women who 
      gave birth to boys. The ratio of cells with the signal to those without the 
      signal ranged from 1 in 144,000 to 1 in 4,000 with a tendency to increase as the 
      pregnancy advanced. The frequency of fetal cells estimated by the PCR experiments 
      was significantly and positively correlated with that found by FISH. The present 
      study suggests that fetal nucleated cells increase in maternal peripheral blood 
      with advancing gestation, from less than 1 in 100,000 nucleated cells in the 
      first trimester to around 1 in 10,000 at term. These frequencies were much lower 
      than those reported by cytological methods.
FAU - Hamada, H
AU  - Hamada H
AD  - Department of Obstetrics and Gynecology, University of Tsukuba, Japan.
FAU - Arinami, T
AU  - Arinami T
FAU - Kubo, T
AU  - Kubo T
FAU - Hamaguchi, H
AU  - Hamaguchi H
FAU - Iwasaki, H
AU  - Iwasaki H
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - DYZ1
MH  - Blood Cell Count
MH  - Blood Specimen Collection
MH  - Cell Separation
MH  - DNA/*blood
MH  - Erythrocytes/cytology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Gestational Age
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Sensitivity and Specificity
MH  - *Y Chromosome
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 10.1007/BF00217766 [doi]
PST - ppublish
SO  - Hum Genet. 1993 Jun;91(5):427-32. doi: 10.1007/BF00217766.