PMID- 8315660
OWN - NLM
STAT- MEDLINE
DCOM- 19930723
LR  - 20091119
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 34
IP  - 6
DP  - 1993 Apr 15
TI  - Characterization of neurotrophin receptors by affinity crosslinking.
PG  - 601-13
AB  - Neurotrophic factors regulate the developmental survival and differentiation of 
      specific neuronal populations. Brain-derived neurotrophic factor (BDNF) and 
      neurotrophin-3 (NT-3) are members of the nerve growth factor (NGF) protein 
      family, also known as the neurotrophins. Insights into the different roles of 
      neurotrophins can be gained by studying the expression of their functional 
      receptors. Here we report the development of procedures for their radiolabeling 
      and efficient crosslinking to specific cell-surface receptors. BDNF and NT-3 
      receptors in cell lines and tissue preparations expressing receptors for the 2 
      neurotrophins have been identified using this affinity crosslinking procedure. 
      Like NGF, BDNF and NT-3 crosslinked to the low affinity NGF receptor (p75NGFR) on 
      PC12 cells. BDNF and NT-3 also crosslinked to cells expressing p145trkB protein, 
      producing an approximately 160 kD neurotrophin-receptor complex. Crosslinking of 
      the 2 neurotrophins in vivo to specific trk family members in many areas of the 
      central nervous system also produced a 160 kD receptor complex. However, in all 
      brain regions a complex of approx. 100 kD could also be identified, all or most 
      of which represents crosslinking to a truncated form of trkB. The broad 
      distribution of BDNF and NT-3 receptors throughout the CNS suggests that 
      neurotrophins may have yet unrecognized functions on specific neuronal 
      populations. BDNF and NT-3 receptors were also found in brain areas in which the 
      neurotrophins themselves are also synthesized, suggesting that beyond long-range 
      trophic effects, these proteins may also act as autocrine or short-range 
      paracrine regulators.
FAU - Escandon, E
AU  - Escandon E
AD  - Department of Neuroscience, Genentech, Inc., South San Francisco, California 
      94080-4990.
FAU - Burton, L E
AU  - Burton LE
FAU - Szonyi, E
AU  - Szonyi E
FAU - Nikolics, K
AU  - Nikolics K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Membrane/metabolism
MH  - Chromatography, Affinity
MH  - Cross-Linking Reagents
MH  - Humans
MH  - Membrane Proteins/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nerve Growth Factors/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurotrophin 3
MH  - PC12 Cells
MH  - Protein-Tyrosine Kinases/biosynthesis
MH  - Rats
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptors, Nerve Growth Factor/*metabolism
MH  - Recombinant Proteins/biosynthesis
EDAT- 1993/04/15 00:00
MHDA- 1993/04/15 00:01
CRDT- 1993/04/15 00:00
PHST- 1993/04/15 00:00 [pubmed]
PHST- 1993/04/15 00:01 [medline]
PHST- 1993/04/15 00:00 [entrez]
AID - 10.1002/jnr.490340602 [doi]
PST - ppublish
SO  - J Neurosci Res. 1993 Apr 15;34(6):601-13. doi: 10.1002/jnr.490340602.