PMID- 8318205
OWN - NLM
STAT- MEDLINE
DCOM- 19930803
LR  - 20190825
IS  - 0886-4470 (Print)
IS  - 0886-4470 (Linking)
VI  - 119
IP  - 7
DP  - 1993 Jul
TI  - Furosemide ototoxicity is enhanced in analbuminemic rats.
PG  - 758-61
AB  - OBJECTIVE: The purpose of this study was to investigate the effect of furosemide 
      on the endocochlear potential (EP) of Sprague-Dawley rats and rats that lack 
      albumin in their serum (Nagase analbuminemic rats [NAR]). DESIGN: Group 
      comparisons between analbuminemic rats and normal Sprague-Dawley rats was carried 
      out, with statistical evaluation using the Student's t test. SETTING: Experiments 
      were carried out in a sound-attenuated booth in a research laboratory. SUBJECTS: 
      Young adult Sprague-Dawley and analbuminemic rats (NAR) 50 to 80 days of age were 
      used as experimental animals. INTERVENTIONS: Subjects were anesthetized with 
      ketamine and xylazine. Furosemide, 35 mg/kg, was injected intravenously in each 
      of three groups: NAR rats, NAR rats pretreated with albumin and normal 
      Sprague-Dawley rats. MAIN OUTCOME MEASURES: Endocochlear potential was measured 
      via the round window membrane approach. Urine samples were collected with a 
      metabolic cage, and volumes were recorded. RESULTS: Sprague-Dawley rats had a 
      very slight EP reduction following furosemide. The NAR rats, however, were found 
      to have an extremely large and rapid reduction of the EP one order of magnitude 
      greater. The NAR rats pretreated with albumin had a significantly smaller 
      reduction of EP than NAR rats not receiving albumin. However, NAR rats pretreated 
      with albumin had a significantly greater urine output than control NAR rats. 
      CONCLUSIONS: These findings support the hypothesis that the access of furosemide 
      to its site of ototoxic action in the cochlea depends on the quantity of unbound 
      furosemide in the serum.
FAU - Rybak, L P
AU  - Rybak LP
AD  - Department of Surgery, Southern Illinois University School of Medicine, 
      Springfield 62794-9230.
FAU - Whitworth, C
AU  - Whitworth C
FAU - Scott, V
AU  - Scott V
LA  - eng
GR  - DC00321/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
RN  - 0 (Serum Albumin)
RN  - 7LXU5N7ZO5 (Furosemide)
SB  - IM
MH  - Action Potentials
MH  - Animals
MH  - Cochlea/*drug effects/physiology
MH  - Cochlear Microphonic Potentials
MH  - Diuresis/drug effects
MH  - Furosemide/*toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/*deficiency
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1001/archotol.1993.01880190054011 [doi]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 1993 Jul;119(7):758-61. doi: 
      10.1001/archotol.1993.01880190054011.