PMID- 8319585
OWN - NLM
STAT- MEDLINE
DCOM- 19930803
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 133
IP  - 1
DP  - 1993 Jul
TI  - Expression of tumor necrosis factor-alpha in mouse spermatogenic cells.
PG  - 389-96
AB  - Enriched fractions of spermatogenic cells were isolated by unit gravity 
      sedimentation and analyzed both for the presence of secreted tumor necrosis 
      factor-alpha (TNF alpha) in vitro by bioassay and for the presence of TNF alpha 
      mRNA by Northern blot analysis. Small quantities of bioactive TNF alpha were 
      consistently detected in medium conditioned by round spermatid fractions. Both 
      pachytene spermatocyte and round spermatid fractions contained RNA that 
      hybridized with murine cDNA probes for TNF alpha, with pachytene spermatocytes 
      containing a normal 1.9-kilobase (kb) transcript, while round spermatids 
      contained principally an approximately 2.8-kb transcript. Both the normal size 
      transcript and the larger haploid-specific transcript were enriched when total 
      RNA from pachytene spermatocyte and round spermatid fractions was passed through 
      an oligo(dT) column. The normal 1.9-kb transcript within pachytene spermatocytes 
      could be induced by exposing the spermatogenic cells to lipopolysaccharides in 
      vitro, yet the approximately 2.8-kb transcript within round spermatids appeared 
      uninduced by LPS treatment. In situ hybridization for the TNF alpha message by 
      using digoxigenin label antisense TNF alpha riboprobe labeled pachytene 
      spermatocytes, round spermatids, and presumptive interstitial macrophages. 
      Spermatogonia and elongating spermatids as well as other interstitial cells were 
      unlabeled or very lightly labeled. Hybridization of 16-day-old prepuberal testis 
      resulted in the labeling of spermatocytes and presumptive interstitial 
      macrophages. RNA from Sertoli cells, but not pachytene spermatocytes or round 
      spermatids, hybridized with human TNF alpha receptor p60 probe in Northern blot 
      analysis. These results are consistent with the working hypothesis that 
      spermatids release TNF alpha, which is detected by Sertoli cells and may serve as 
      a paracrine factor, regulating an as yet unidentified process in spermatogenesis.
FAU - De, S K
AU  - De SK
AD  - Department of Anatomy and Cell Biology, University of Kansas Medical Center, 
      Kansas City 66160-7400.
FAU - Chen, H L
AU  - Chen HL
FAU - Pace, J L
AU  - Pace JL
FAU - Hunt, J S
AU  - Hunt JS
FAU - Terranova, P F
AU  - Terranova PF
FAU - Enders, G C
AU  - Enders GC
LA  - eng
GR  - BRSG S07-RR-5373/RR/NCRR NIH HHS/United States
GR  - HD-29156/HD/NICHD NIH HHS/United States
GR  - R29-HD-26639/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - *Gene Expression
MH  - In Situ Hybridization
MH  - Leydig Cells/chemistry
MH  - Liver/chemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - RNA, Messenger/analysis
MH  - Sertoli Cells/chemistry
MH  - Spermatids/chemistry/metabolism
MH  - Spermatozoa/chemistry/*metabolism
MH  - Testis/chemistry
MH  - Tumor Necrosis Factor-alpha/analysis/*genetics
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1210/endo.133.1.8319585 [doi]
PST - ppublish
SO  - Endocrinology. 1993 Jul;133(1):389-96. doi: 10.1210/endo.133.1.8319585.