PMID- 8319773
OWN - NLM
STAT- MEDLINE
DCOM- 19930804
LR  - 20061115
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 207
IP  - 1
DP  - 1993 Jul
TI  - Reversion and cell hybridization of a dedifferentiated rat hepatoma cell: 
      dissociation of mechanisms establishing the hepatic traits.
PG  - 48-56
AB  - The expression of a mouse serum albumin (MSA) gene introduced into differentiated 
      and dedifferentiated variant cells of a rat hepatoma line depends on the overall 
      differentiated state of the recipient cell. Thus, the transcription rate of this 
      mouse gene is very high in differentiated cells compared to that in the variant. 
      Furthermore, the activation of its expression is observed during the course of 
      reversion of the variant cell. In well-differentiated hybrids between the variant 
      and cells of the original line, the expression of the MSA gene depends on the 
      phenotype of the transfected parental cell: transcription of this foreign gene is 
      detected only in hybrids in which it was initially integrated into the genome of 
      the differentiated parent. Gene dosage effects are not involved as the copy 
      number of the MSA gene is identical in both types of transfected cells. These 
      results demonstrate that the establishment of the liver phenotype in 
      differentiated hybrids and revertants is due to different mechanisms.
FAU - Ng, K H
AU  - Ng KH
AD  - INSERM U347, Le Kremlin-Bicetre, France.
FAU - Maigne, J
AU  - Maigne J
FAU - Fougere-Deschatrette, C
AU  - Fougere-Deschatrette C
FAU - Poupon, M F
AU  - Poupon MF
FAU - Deschatrette, J
AU  - Deschatrette J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Clone Cells
MH  - *Gene Expression Regulation, Neoplastic
MH  - Hybrid Cells
MH  - Liver Neoplasms, Experimental/*genetics
MH  - Mice
MH  - Phenotype
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Serum Albumin/biosynthesis/*genetics
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - S0014-4827(83)71161-4 [pii]
AID - 10.1006/excr.1993.1161 [doi]
PST - ppublish
SO  - Exp Cell Res. 1993 Jul;207(1):48-56. doi: 10.1006/excr.1993.1161.