PMID- 8321117
OWN - NLM
STAT- MEDLINE
DCOM- 19930805
LR  - 20061115
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 14
IP  - 1
DP  - 1993 Jan
TI  - Salmonella abortusovis infection in susceptible BALB/cby mice: importance of 
      Lyt-2+ and L3T4+ T cells in acquired immunity and granuloma formation.
PG  - 45-55
AB  - The role of T cells in granulomatous responses and in acquired immunity against 
      Salmonella abortusovis (SAO) infection was studied in a murine model. Mice were 
      subcutaneously (s.c.) vaccinated with a live attenuated strain of SAO. One month 
      after vaccination, the transfer of primed spleen cells (1 x 10(8) cells per 
      mouse) to syngeneic recipient mice conferred a significant protection of 3 log10, 
      measured by spleen colonization on day 6 after s.c. challenge. In vitro treatment 
      of spleen cells, before the transfer, with anti-Lyt-2 monoclonal antibody (IgG2b 
      isotype MAb) and complement significantly impaired the protective activity. 
      Treatment with anti-L3T4 MAb also diminished transferred protection, but to a 
      lesser degree. Depletion of both L3T4+ and Lyt-2+ T cells completely abrogated 
      protection. MAb treatment of spleen cells in vitro did not seem to have any 
      effect on antibody response in recipient mice. Six days after the challenge 
      protected recipient mice showed organized granulomas in the liver containing 
      Mac-1+ macrophages and L3T4+ T cells. In non-protected mice at 6 days 
      post-challenge, large infiltrates of T lymphocytes and macrophages were observed, 
      but as numerous lesions with necrosis of hepatocytes; no granuloma were seen. In 
      our experimental conditions, Lyt-2+ and L3T4+ T cells appeared to play, alone and 
      in synergy, a role in vaccine-induced immunity against SAO and hepatic granulomas 
      may contribute to the control of the infection.
FAU - Guilloteau, L
AU  - Guilloteau L
AD  - Unite Genetique et Immunite, Institut National de la Recherche Agronomique, 
      Centre de Tours, Nouzilly, France.
FAU - Buzoni-Gatel, D
AU  - Buzoni-Gatel D
FAU - Bernard, F
AU  - Bernard F
FAU - Lantier, I
AU  - Lantier I
FAU - Lantier, F
AU  - Lantier F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Antigens, Ly)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Vaccines, Attenuated)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - *Antigens, Differentiation, T-Lymphocyte
MH  - *Antigens, Ly
MH  - Bacterial Vaccines
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Granuloma/immunology/*microbiology
MH  - Immunotherapy, Adoptive
MH  - Liver Diseases/immunology/*microbiology
MH  - Mice
MH  - Mice, Inbred BALB C/genetics/*immunology
MH  - Salmonella/immunology/*physiology
MH  - Salmonella Infections, Animal/genetics/*immunology/microbiology
MH  - Spleen/cytology
MH  - T-Lymphocyte Subsets/*immunology
MH  - Vaccination
MH  - Vaccines, Attenuated
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - S0882-4010(83)71005-3 [pii]
AID - 10.1006/mpat.1993.1005 [doi]
PST - ppublish
SO  - Microb Pathog. 1993 Jan;14(1):45-55. doi: 10.1006/mpat.1993.1005.