PMID- 8326010
OWN - NLM
STAT- MEDLINE
DCOM- 19930812
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 92
IP  - 1
DP  - 1993 Jul
TI  - Relationship between skeletal muscle lipoprotein lipase activity and 24-hour 
      macronutrient oxidation.
PG  - 441-5
AB  - A low ratio of whole-body 24-h fat/carbohydrate (CHO) oxidation has been shown to 
      be a predictor of subsequent body weight gain. We tested the hypothesis that the 
      variability of this ratio may be related to differences in skeletal muscle 
      metabolism. Since lipoprotein lipase (LPL) plays a pivotal role in partitioning 
      lipoprotein-borne triglycerides to adipose (storage) and skeletal muscle (mostly 
      oxidation), we postulated that a low ratio of fat/CHO oxidation was associated 
      with a low skeletal muscle LPL (SMLPL) activity. As an index of substrate 
      oxidation, 24-h RQ was measured under sedentary and eucaloric conditions in 16 
      healthy nondiabetic Pima males. During a 6-h euglycemic, hyperinsulinemic clamp, 
      muscle biopsies were obtained at baseline, 3, and 6 h. Heparin-elutable SMLPL 
      activity was 2.92 +/- 0.56 nmol free fatty acids/g.min (mean +/- SD) at baseline, 
      was unchanged (2.91 +/- 0.51) at the third hour, and increased significantly (P < 
      0.05) to 3.13 +/- 0.57 at the sixth hour of the clamp. The mean (of baseline and 
      3-h) SMLPL activity correlated inversely with 24-h RQ (r = 0.57, P < 0.03) but 
      not with body size, body composition, or insulin-mediated glucose uptake. Since 
      SMLPL activity is related to the ratio of whole body fat/CHO oxidation rate, a 
      decreased muscle LPL activity may, therefore, predispose to obesity.
FAU - Ferraro, R T
AU  - Ferraro RT
AD  - Clinical Diabetes and Nutrition Section, National Institute of Diabetes and 
      Digestive and Kidney Diseases, National Institutes of Health, Phoenix, Arizona 
      85016.
FAU - Eckel, R H
AU  - Eckel RH
FAU - Larson, D E
AU  - Larson DE
FAU - Fontvieille, A M
AU  - Fontvieille AM
FAU - Rising, R
AU  - Rising R
FAU - Jensen, D R
AU  - Jensen DR
FAU - Ravussin, E
AU  - Ravussin E
LA  - eng
GR  - DK-26354/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Insulin)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
SB  - IM
MH  - Carbohydrate Metabolism
MH  - Energy Metabolism
MH  - Humans
MH  - Indians, North American
MH  - Insulin/physiology
MH  - Lipid Metabolism
MH  - Lipoprotein Lipase/*metabolism
MH  - Male
MH  - Muscles/*metabolism
PMC - PMC293630
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
PMCR- 1993/07/01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 10.1172/JCI116586 [doi]
PST - ppublish
SO  - J Clin Invest. 1993 Jul;92(1):441-5. doi: 10.1172/JCI116586.