PMID- 8330265
OWN - NLM
STAT- MEDLINE
DCOM- 19930817
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 67
IP  - 2
DP  - 1993 Jun
TI  - Karyotype and FISH analysis of a newly established cell line derived from a human 
      bladder carcinoma.
PG  - 101-7
AB  - A new human malignant urologic cell line was established in vitro from a 
      moderately differentiated transitional cell carcinoma of the bladder and 
      cytogenetically characterized. Repeated chromosome analyses of the cell line 
      using conventional RHG and GTG banding and non-radioactive in situ hybridization 
      showed a stable karyotype with a modal number of 48 and chromosomal 
      rearrangements, some of which have not been previously described. Numerical 
      deviation included three trisomies (+7, +8, +9) and one nullisomy (-19, -19). 
      Structural changes involved a balanced translocation (1;5)(q12;q12), an 
      isochromosome 3q, a 14p+, and two markers. Fluorescence in situ hybridization 
      (FISH), using biotin-labeled alpha satellite probes for chromosome 9 or painting 
      for chromosomes 1 and 8, applied to interphase nuclei or metaphases showed 
      similar results to those found by conventional cytogenetic study. This cell line 
      may be an interesting model for fuller characterization by molecular biology 
      studies and for testing anti-cancer drugs in vitro.
FAU - Perissel, B
AU  - Perissel B
AD  - Laboratoire d'Histologie-Embryologie-Cytogenetique, Faculte de Medecine de 
      Clermont-Ferrand, France.
FAU - Benkhalifa, M
AU  - Benkhalifa M
FAU - Taillandier, J
AU  - Taillandier J
FAU - Dastugue, N
AU  - Dastugue N
FAU - Haus, O
AU  - Haus O
FAU - Charbonne, F
AU  - Charbonne F
FAU - Giraud, B
AU  - Giraud B
FAU - Malet, P
AU  - Malet P
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Carcinoma, Transitional Cell/*genetics
MH  - Cell Line
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 0165-4608(93)90160-N [pii]
AID - 10.1016/0165-4608(93)90160-n [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1993 Jun;67(2):101-7. doi: 10.1016/0165-4608(93)90160-n.