PMID- 8330537
OWN - NLM
STAT- MEDLINE
DCOM- 19930817
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 117
IP  - 2
DP  - 1993 Feb
TI  - achaete-scute feminizing activities and Drosophila sex determination.
PG  - 737-49
AB  - Sex determination in Drosophila depends on X-linked 'numerator' genes activating 
      early Sex-lethal (Sxl) transcription in females. One numerator gene, sisterless-b 
      (sis-b), corresponds to the achaete-scute (AS-C) T4 basic-helix-loop-helix (bHLH) 
      gene. Two other closely related AS-C bHLH genes, T3 and T5, appear not to 
      function as numerator elements. We analyzed endogenous AS-C expression and show 
      that T4 is the major AS-C numerator gene because it is expressed earlier and more 
      strongly than are T3 and T5. Only T4 expression is detectable during the early 
      syncytial stages when Sxl state is being determined. Nevertheless, the effects of 
      ectopic AS-C gene expression show that T3 and T5 proteins display weak but 
      significant feminizing activities, enhancing male-lethality, and rescuing the 
      female-lethality of sis mutations. Detailed examination of Sxl expression in 
      rescued embryos suggests that female cells may be viable in the absence of 
      detectable Sxl protein expression.
FAU - Parkhurst, S M
AU  - Parkhurst SM
AD  - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, 
      Washington 98104.
FAU - Lipshitz, H D
AU  - Lipshitz HD
FAU - Ish-Horowicz, D
AU  - Ish-Horowicz D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
SB  - IM
GS  - Sxl
GS  - hb-T4
GS  - hb-T5
GS  - hb-h
GS  - sis-b
MH  - Animals
MH  - *Dosage Compensation, Genetic
MH  - Drosophila/embryology/*genetics
MH  - Female
MH  - Gene Expression/*genetics
MH  - *Genes, Insect
MH  - Genes, Regulator/*genetics
MH  - *Sex Determination Analysis
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1242/dev.117.2.737 [doi]
PST - ppublish
SO  - Development. 1993 Feb;117(2):737-49. doi: 10.1242/dev.117.2.737.