PMID- 8332448
OWN - NLM
STAT- MEDLINE
DCOM- 19930813
LR  - 20071115
VI  - 35
IP  - 2
DP  - 1993 Apr
TI  - Trisomy 12 in B-cell chronic lymphocytic leukemia: interphase study by in situ 
      hybridization in 75 patients.
PG  - 129-34
AB  - Trisomy 12 is the most common cytogenetic abnormality in chronic lymphocytic 
      leukaemia (CLL) and may be a prognostic indicator. In the present study, 
      fluorescence in situ hybridization (FISH) is shown to be a method of choice for 
      detection of trisomy 12 in interphase cells. Seventy-five cases of B-cell CLL 
      were analysed with a chromosome 12 specific alpha satellite DNA probe and results 
      compared with those from cytogenetic analysis. FISH showed the three 
      hybridization spots characteristic of trisomy 12 in 32/75 patients (42.6%). 
      Sixty-three patients were also studied by conventional cytogenetics: failure in 7 
      cases, normal karyotype in 28, trisomy 12 in 9 (14.3%) and in 19 cases 
      abnormalities other than trisomy 12. In these same 63 patients, trisomy 12 was 
      detected on 29 occasions by FISH (46%): in one case of failure by cytogenetic 
      analysis, in 9 cases thought to have a normal karyotype, in 10 cases carrying 
      abnormalities other than trisomy 12 and in all 9 cases showing trisomy 12 by 
      conventional cytogenetic investigation. Correlation between trisomy 12 and the 
      three stages of the Binet classification indicated an increasing proportion of 
      trisomy 12 from stage A to stage C. It is concluded that fluorescence in situ 
      hybridization is a powerful and sensitive technique for detection of trisomy 12 
      in CLL and although more cases will be required to confirm a correlation between 
      the incidence of trisomy 12 and the stage of the disease, this link could be 
      important from a prognostic point of view.
FAU - Coignet, L
AU  - Coignet L
AD  - Departement d'Hematologie, Hopital Nord, Saint-Priest-en-Jarez, France.
FAU - Bertheas, M F
AU  - Bertheas MF
FAU - Vasselon, C
AU  - Vasselon C
FAU - Jaubert, J
AU  - Jaubert J
FAU - Reynaud, J
AU  - Reynaud J
FAU - Calmard-Oriol, P
AU  - Calmard-Oriol P
FAU - Brizard, C P
AU  - Brizard CP
FAU - Guyotat, D
AU  - Guyotat D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Nouv Rev Fr Hematol (1978)
JT  - Nouvelle revue francaise d'hematologie
JID - 7909092
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosomes, Human, Pair 12
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Interphase/*genetics
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Trisomy
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PST - ppublish
SO  - Nouv Rev Fr Hematol (1978). 1993 Apr;35(2):129-34.