PMID- 8333833
OWN - NLM
STAT- MEDLINE
DCOM- 19930817
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 194
IP  - 1
DP  - 1993 Jul 15
TI  - Interleukin-1 induction of tumor necrosis factor-alpha mRNA and bioactive tumor 
      necrosis factor-alpha in a pancreatic beta-cell line by a mechanism requiring no 
      de novo protein synthesis.
PG  - 163-9
AB  - Interleukin-1 (IL-1) has been implicated as an effector in insulitis of Type 1 
      (insulin-dependent) diabetes. Exposure of a beta-cell line (beta TC1) to IL-1 
      beta resulted in an increase of preproinsulin mRNA at 0.5 h followed by a gradual 
      decrease. Tumor necrosis factor-alpha (TNF-alpha) mRNA expression by beta TC1 
      cells was demonstrated 1-3 h after the addition of IL-1 beta. TNF bioactivity was 
      detected in homogenates of beta TC1 cells exposed to IL-1. The supplementation of 
      cycloheximide (CHX) together with IL-1 beta resulted in the superinduction of 
      TNF-alpha mRNA, suggesting that de novo protein synthesis is not required in 
      IL-1-induced TNF-alpha mRNA expression. Endogenous TNF-alpha of beta-cells may be 
      involved in the islet lesion of type 1 diabetes.
FAU - Takane, N
AU  - Takane N
AD  - Department of Medicine, Kurume University School of Medicine, Fukuoka, Japan.
FAU - Yamada, K
AU  - Yamada K
FAU - Otabe, S
AU  - Otabe S
FAU - Inoue, M
AU  - Inoue M
FAU - Nonaka, K
AU  - Nonaka K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Actins)
RN  - 0 (Insulin)
RN  - 0 (Interleukin-2)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 61116-24-3 (preproinsulin)
RN  - 9035-68-1 (Proinsulin)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Actins/biosynthesis
MH  - Animals
MH  - Blotting, Northern
MH  - Cycloheximide/pharmacology
MH  - Gene Expression/*drug effects
MH  - Humans
MH  - Insulin
MH  - Interleukin-2/*pharmacology
MH  - Islets of Langerhans
MH  - Kinetics
MH  - Mice
MH  - Proinsulin/biosynthesis
MH  - Protein Precursors/biosynthesis
MH  - RNA, Messenger/*biosynthesis
MH  - Recombinant Proteins/pharmacology
MH  - Transcription, Genetic/drug effects
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1993/07/15 00:00
MHDA- 1993/07/15 00:01
CRDT- 1993/07/15 00:00
PHST- 1993/07/15 00:00 [pubmed]
PHST- 1993/07/15 00:01 [medline]
PHST- 1993/07/15 00:00 [entrez]
AID - S0006-291X(83)71799-7 [pii]
AID - 10.1006/bbrc.1993.1799 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1993 Jul 15;194(1):163-9. doi: 
      10.1006/bbrc.1993.1799.