PMID- 8335403
OWN - NLM
STAT- MEDLINE
DCOM- 19930824
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 54
IP  - 6
DP  - 1993 Jul 30
TI  - Infection with a transforming growth factor alpha anti-sense retroviral 
      expression vector reduces the in vitro growth and transformation of a human colon 
      cancer cell line.
PG  - 952-8
AB  - Transforming growth factor alpha (TGF alpha) is a growth factor produced by colon 
      cancer cells which may function as an autocrine growth regulator. Therefore, the 
      proliferation and transformation of colon cancer cells might be attenuated by 
      blocking the production of endogenous TGF alpha. GEO cells, from a human colon 
      carcinoma cell line that expresses TGF alpha and functional epidermal growth 
      factor (EGF) receptors, were infected with a replication-defective, recombinant 
      amphotropic retroviral expression vector containing the neomycin-resistance gene 
      and a 435-bp ApaI-EcoRI coding fragment of the human TGF alpha cDNA oriented in 
      the 3' to 5' direction under the transcriptional control of the 
      heavy-metal-inducible mouse metallothionein I promoter. Following antibiotic 
      selection, G418-resistant colonies were pooled and expanded into a cell line (GEO 
      TGF alpha AS cells). A 50 to 70% inhibition in the production of secreted and 
      cell-associated TGF alpha protein was observed in GEO TGF alpha AS cells that had 
      been maintained in CdCl2-supplemented medium. Moreover, a growth inhibition of 
      70% and 50% was observed in CdCl2-treated GEO TGF alpha AS cells under 
      anchorage-dependent and anchorage-independent culture conditions, respectively. 
      In contrast, CdCl2 treatment of parental GEO cells had no significant effect upon 
      these parameters. Our results suggest that TGF alpha may be involved in 
      modulating the in vitro cell growth and transformation of human colon cancer 
      cells that express both this growth factor and its cognate receptor.
FAU - Ciardiello, F
AU  - Ciardiello F
AD  - Cattedra di Oncologia Medica, II Facolta di Medicina e Chirurgia, Universita 
      degli Studi di Napoli, Italy.
FAU - Bianco, C
AU  - Bianco C
FAU - Normanno, N
AU  - Normanno N
FAU - Baldassarre, G
AU  - Baldassarre G
FAU - Pepe, S
AU  - Pepe S
FAU - Tortora, G
AU  - Tortora G
FAU - Bianco, A R
AU  - Bianco AR
FAU - Salomon, D S
AU  - Salomon DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (RNA, Antisense)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Animals
MH  - Cell Division/physiology
MH  - *Cell Transformation, Neoplastic
MH  - Colonic Neoplasms/*pathology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - RNA, Antisense
MH  - RNA, Messenger
MH  - Radioimmunoassay
MH  - Retroviridae/genetics
MH  - Transforming Growth Factor alpha/*physiology
MH  - Tumor Cells, Cultured
EDAT- 1993/07/30 00:00
MHDA- 1993/07/30 00:01
CRDT- 1993/07/30 00:00
PHST- 1993/07/30 00:00 [pubmed]
PHST- 1993/07/30 00:01 [medline]
PHST- 1993/07/30 00:00 [entrez]
AID - 10.1002/ijc.2910540615 [doi]
PST - ppublish
SO  - Int J Cancer. 1993 Jul 30;54(6):952-8. doi: 10.1002/ijc.2910540615.