PMID- 833541
OWN - NLM
STAT- MEDLINE
DCOM- 19770331
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 72
IP  - 1
DP  - 1977 Jan
TI  - States of activation of chick kidney adenylate cyclase induced by parathyroid 
      hormone and guanyl nucleotides.
PG  - 69-79
AB  - Several aspects of the activation of adenylate cyclase by guanosine 
      5'-triphosphate (GTP), 5'-guanylylimidodiphosphate (Gpp(NH)p) and bovine 
      parathyroid hormone (bPTH) have been studied in chick kidney plasma membrane 
      preparations. GTP (10(-4) mol/l), Gpp(NH)p (10(-4) mol/l) and bPTH (10 i.u./ml) 
      activated adenylate cyclase without any significant time lag. However a 2 min 
      delay was observed before the activity of the enzyme increased after the addition 
      of bPTH (-6 leads to +34) to incubations. The early (0-3 min) effects of GTP and 
      Gpp(NH)p upon chick kidney adenylate cyclase activity were antagonized by the 
      addition of the alternative guanyl nucleotide. After 5 min of incubation with 
      kidney plasma membranes, Gpp(NH)p induced a stable state of activation of 
      adenylate cyclase which was not reversible by subsequent addition of GTP. GTP did 
      not induce an irreversible state of enzyme activation. In pre-incubation studies, 
      GTP did not produce a persistent enzyme activation and did not modify the effect 
      of Gpp(NH)p added subsequently at the incubation stage. Gpp(NH)p produced a 
      stable state of activation of adenylate cyclase which was not inhibited by 
      addition of GTP at the incubation stage. Bovine PTH (2-34) inhibited the effect 
      of bPTH upon adenylate cyclase activity when the native hormone (10 i.u./ml) had 
      been incubated with plasma membranes for up to 8 min before addition of the 
      analogue (5 mug/ml). Incubation of plasma membranes with bPTH (2-34) for as 
      little as 10 s prevented activation of adenylate cyclase by subsequent addition 
      of bPTH. This pattern was confirmed in pre-incubation studies. After 
      pre-incubation of kidney membranes with bPTH and bPTH (2-34), followed by 
      washing, an acid extract of the membranes contained immunoreactive bPTH. Gpp(NH)p 
      produced a greater increase in adenylate cyclase activity in membranes 
      pre-incubated with bPTH or bPTH (2-34) than in membranes pre-incubated with 
      buffer alone, suggesting that the hormone and analogue facilitated the 
      interaction of Gpp(NH)p with adenylate cyclase.
FAU - Michalangeli, V P
AU  - Michalangeli VP
FAU - Hunt, N H
AU  - Hunt NH
FAU - Martin, T J
AU  - Martin TJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Hormones)
RN  - 0 (Parathyroid Hormone)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*biosynthesis
MH  - Animals
MH  - Cell Membrane/drug effects/enzymology
MH  - Chick Embryo
MH  - Enzyme Induction
MH  - Guanine Nucleotides/*pharmacology
MH  - Guanosine Triphosphate/*pharmacology
MH  - Hormones/pharmacology
MH  - Kidney/drug effects/*enzymology
MH  - Parathyroid Hormone/*pharmacology
EDAT- 1977/01/01 00:00
MHDA- 1977/01/01 00:01
CRDT- 1977/01/01 00:00
PHST- 1977/01/01 00:00 [pubmed]
PHST- 1977/01/01 00:01 [medline]
PHST- 1977/01/01 00:00 [entrez]
AID - 10.1677/joe.0.0720069 [doi]
PST - ppublish
SO  - J Endocrinol. 1977 Jan;72(1):69-79. doi: 10.1677/joe.0.0720069.