PMID- 8335975
OWN - NLM
STAT- MEDLINE
DCOM- 19930824
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 168
IP  - 2
DP  - 1993 Aug
TI  - Proton magnetic resonance spectroscopy of polymorphonuclear leukocytes from 
      patients with serious bacterial infections.
PG  - 386-92
AB  - Triacylglycerols in human neutrophils exposed to proinflammatory stimuli generate 
      a high-resolution proton magnetic resonance (1H MR) spectrum. Lipid cross-peak F 
      volumes in neutrophils from patients with inflammatory conditions were measured. 
      Values in patients hospitalized with localized infections (14.4 +/- 9.0; mean +/- 
      SD) or bacteremia (19.3 +/- 9.7) were significantly higher than in patients with 
      noninflammatory conditions (6.2 +/- 5.3) and healthy controls (2.0 +/- 3.0; P < 
      .001). The positive predictive value of F volumes > 10 was 93% for all infection; 
      the negative predictive value of volumes < or = 10 was 68% for all infection and 
      92% for bacteremia. Plasma lipopolysaccharide (LPS) concentrations were highest 
      in bacteremic patients but did not correlate with levels of tumor necrosis 
      factor-alpha (TNF alpha) or interleukin-6. In vitro, LPS increased F volumes of 
      control neutrophils from 2.0 +/- 3.0 to 37.2 +/- 6.7 (P < .001); TNF alpha had no 
      effect. F volumes in 1H MR spectra may be useful clinically to discriminate 
      between serious bacterial infection and other inflammatory conditions. TNF alpha 
      is not the stimulus for generation of lipid spectra in vivo.
FAU - May, G L
AU  - May GL
AD  - Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead 
      Hospital, Australia.
FAU - Sztelma, K
AU  - Sztelma K
FAU - Paul, M L
AU  - Paul ML
FAU - Sorrell, T C
AU  - Sorrell TC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Bacterial Infections/*blood
MH  - Humans
MH  - Interleukin-6/blood
MH  - Lipopolysaccharides/blood/pharmacology
MH  - Magnetic Resonance Imaging
MH  - Neutrophils/*chemistry
MH  - Tumor Necrosis Factor-alpha/analysis/pharmacology
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - 10.1093/infdis/168.2.386 [doi]
PST - ppublish
SO  - J Infect Dis. 1993 Aug;168(2):386-92. doi: 10.1093/infdis/168.2.386.