PMID- 8338951
OWN - NLM
STAT- MEDLINE
DCOM- 19930902
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 82
IP  - 3
DP  - 1993 Aug 1
TI  - In situ hybridization on May-Grunwald Giemsa-stained bone marrow and blood smears 
      of patients with hematologic disorders allows detection of cell-lineage-specific 
      cytogenetic abnormalities.
PG  - 884-8
AB  - Bone marrow and blood from patients with acute myeloid leukemia and 
      myelodysplastic syndrome were studied by simultaneous analysis of cell morphology 
      and karyotype. A combined technique of May-Grunwald Giemsa (MGG) for cell 
      morphology and fluorescence in situ hybridization (FISH) with chromosome-specific 
      DNA probes for detection of cytogenetic aberrations allowed us to investigate 
      cell-lineage-specific chromosomal abnormalities. We introduced video recordings 
      to examine large numbers of cells. Briefly, evaluation was first performed on MGG 
      slides, during which cell position and morphology were recorded on an S-VHS 
      recorder. Subsequently, the same slides were used for FISH. This resulted in the 
      identification of MGG-stained cells on the video screen and, at the same time, 
      the interpretation of FISH signals in the fluorescence microscope. Specimens of 
      bone marrow or blood samples from four patients with different hematologic 
      malignancies were studied. One of these patients was studied before and after 
      cytotoxic treatment. The gain or loss of chromosomes could be detected easily and 
      morphologically assigned to the blasts in all patients and to a variable 
      proportion of the myelomonocytic lineage in two patients, but not to the 
      lymphocytes. Thus, this method provides new possibilities for investigating the 
      clonality of hematologic malignancies.
FAU - van Lom, K
AU  - van Lom K
AD  - Department of Hematology, University Hospital, Rotterdam, The Netherlands.
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Smit, E M
AU  - Smit EM
FAU - Lowenberg, B
AU  - Lowenberg B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Acute Disease
MH  - Bone Marrow/*pathology
MH  - Chromosome Aberrations/*diagnosis
MH  - Chromosome Disorders
MH  - Female
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Leukemia, Myeloid/genetics/*pathology
MH  - Male
MH  - Myelodysplastic Syndromes/genetics/*pathology
MH  - Video Recording
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - S0006-4971(20)82374-X [pii]
PST - ppublish
SO  - Blood. 1993 Aug 1;82(3):884-8.