PMID- 8344430
OWN - NLM
STAT- MEDLINE
DCOM- 19930909
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 328
IP  - 1-2
DP  - 1993 Aug 9
TI  - Proteolytic processing of the hepatocyte growth factor/scatter factor receptor by 
      furin.
PG  - 25-9
AB  - The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an 
      alpha- and a beta-subunit, which are derived from a single-chain precursor by 
      endoproteolytic processing. The precursor is not proteolytically processed in 
      LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells 
      was cleaved in vitro by furin. Furthermore, the HGF/SF receptor was 
      proteolytically processed in LoVo cells transfected with furin cDNA. These 
      results indicate that furin is a processing endoprotease for the HGF/SF receptor. 
      Tyrosine autophosphorylation of the uncleaved receptor was induced by HGF/SF, and 
      the growth of the cells expressing the uncleaved receptor was stimulated by 
      HGF/SF, indicating that the proteolytic processing of the receptor is not 
      essential for the signal transduction of HGF/SF.
FAU - Komada, M
AU  - Komada M
AD  - Institute for Liver Research, Kansai Medical University, Osaka, Japan.
FAU - Hatsuzawa, K
AU  - Hatsuzawa K
FAU - Shibamoto, S
AU  - Shibamoto S
FAU - Ito, F
AU  - Ito F
FAU - Nakayama, K
AU  - Nakayama K
FAU - Kitamura, N
AU  - Kitamura N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.21.- (Subtilisins)
RN  - EC 3.4.21.75 (Furin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - CHO Cells
MH  - Cricetinae
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Endopeptidases/metabolism
MH  - Furin
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - Signal Transduction
MH  - Subtilisins/genetics/*pharmacology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1993/08/09 00:00
MHDA- 1993/08/09 00:01
CRDT- 1993/08/09 00:00
PHST- 1993/08/09 00:00 [pubmed]
PHST- 1993/08/09 00:01 [medline]
PHST- 1993/08/09 00:00 [entrez]
AID - 0014-5793(93)80958-W [pii]
AID - 10.1016/0014-5793(93)80958-w [doi]
PST - ppublish
SO  - FEBS Lett. 1993 Aug 9;328(1-2):25-9. doi: 10.1016/0014-5793(93)80958-w.