PMID- 8345216
OWN - NLM
STAT- MEDLINE
DCOM- 19930903
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 101
IP  - 2
DP  - 1993 Aug
TI  - Mast cell degranulation upregulates alpha 6 integrins on epidermal Langerhans 
      cells.
PG  - 150-4
AB  - The expression of the alpha 6 beta 4 and alpha 6 beta 1 integrins on epidermal 
      Langerhans cells (LC) before and after mast cell degranulation was studied in 
      cultured human neonatal foreskin by immunohistochemistry. Twenty-four hours after 
      addition of mast cell secretagogues, morphine sulfate, or substance P, solitary 
      mid-epidermal cells showed staining for the integrin subunits alpha 6, beta 4, 
      and beta 1. This expression was not observed in cultured control explants, and 
      immunostained cells were confirmed to be non-epithelial, dendritic cells by 
      immuno-electron microscopy. The identity of these cells as LC was further 
      established by coincident staining for alpha 6 and CD1a using double 
      immunofluorescence labeling. Addition of tumor necrosis factor-alpha (TNF alpha), 
      the predominant cytokine in mast cell granules, also induced LC to express alpha 
      6 integrins. Furthermore, preincubation of skin organ cultures with anti-TNF 
      alpha antibodies or the mast cell inhibitor cromolyn sodium abrogated the ability 
      to induce alpha 6 integrins on LC consequent to experimental mast cell 
      degranulation by substance P. These data implicate a role for mast cell-derived 
      TNF alpha in the regulation of the integrins alpha 6 beta 4 and alpha 6 beta 1 on 
      LC. These findings may have important implications relevant to mechanisms for 
      spatial localization of LC within the cutaneous compartments during immune 
      responses.
FAU - Ioffreda, M D
AU  - Ioffreda MD
AD  - Department of Dermatology, University of Pennsylvania School of Medicine, 
      Philadelphia 19104.
FAU - Whitaker, D
AU  - Whitaker D
FAU - Murphy, G F
AU  - Murphy GF
LA  - eng
GR  - AR39674/AR/NIAMS NIH HHS/United States
GR  - CA40358/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Integrins)
SB  - IM
MH  - Cell Degranulation/*physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant, Newborn
MH  - Integrins/*physiology
MH  - Keratinocytes/ultrastructure
MH  - Langerhans Cells/*chemistry/ultrastructure
MH  - Male
MH  - Mast Cells/*cytology
MH  - Microscopy, Immunoelectron
MH  - Up-Regulation
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - S0022-202X(93)90431-G [pii]
AID - 10.1111/1523-1747.ep12363632 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1993 Aug;101(2):150-4. doi: 10.1111/1523-1747.ep12363632.