PMID- 8349412
OWN - NLM
STAT- MEDLINE
DCOM- 19930910
LR  - 20131121
IS  - 0367-8377 (Print)
IS  - 0367-8377 (Linking)
VI  - 41
IP  - 6
DP  - 1993 Jun
TI  - Recombinant human brain-derived neurotrophic factor (rHuBDNF). Disulfide 
      structure and characterization of BDNF expressed in CHO cells.
PG  - 548-52
AB  - Three disulfide linkages of recombinant human brain-derived neurotrophic factor 
      (BDNF) were determined by peptide sequence analysis and characterized by mass 
      spectrometry. The three disulfide bonds for BDNF expressed in Chinese hamster 
      ovary cells include Cys-13-Cys-80, Cys-58-Cys-109 and Cys-68-Cys-111, and the 
      disulfide structure was homologous to that of nerve growth factor.
FAU - Acklin, C
AU  - Acklin C
AD  - Department of Protein Structure, Amgen Inc., Thousand Oaks, California.
FAU - Stoney, K
AU  - Stoney K
FAU - Rosenfeld, R A
AU  - Rosenfeld RA
FAU - Miller, J A
AU  - Miller JA
FAU - Rohde, M F
AU  - Rohde MF
FAU - Haniu, M
AU  - Haniu M
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Int J Pept Protein Res
JT  - International journal of peptide and protein research
JID - 0330420
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 48TCX9A1VT (Cystine)
RN  - EC 3.4.24.27 (Thermolysin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - CHO Cells
MH  - Cricetinae
MH  - Cystine/chemistry
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*chemistry/metabolism
MH  - Nerve Tissue Proteins/*chemistry/metabolism
MH  - Recombinant Proteins/chemistry
MH  - Sequence Analysis
MH  - Thermolysin/metabolism
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PST - ppublish
SO  - Int J Pept Protein Res. 1993 Jun;41(6):548-52.