PMID- 8351158
OWN - NLM
STAT- MEDLINE
DCOM- 19930916
LR  - 20210112
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 53
IP  - 3
DP  - 1993 Jun
TI  - Magnesium sulphate injected subcutaneously suppresses autotomy in peripherally 
      deafferented rats.
PG  - 287-293
LID - 10.1016/0304-3959(93)90225-E [doi]
AB  - In rats, recent evidence suggests that injury discharge caused by peripheral 
      nerve section releases excitatory amino acids into the spinal cord which in turn 
      influences decisively the development of autotomy, a self-mutilation behaviour 
      directed towards the denervated areas. Autotomy has been proposed as a 
      behavioural correlate of the neuropathic pain which occurs in humans after 
      complete nerve lesions. Mg2+ ions have been shown to offer protection from 
      neurological and degenerative disorders in which excitatory amino acids are 
      putatively involved. To ascertain the preventive value of Mg2+ administration on 
      autotomy, male rats underwent unilateral ligation and transection of the sciatic 
      and saphenous nerves 30 min after being injected subcutaneously (s.c.) with 300 
      or 600 mg/kg MgSO4 or saline. Thereafter, autotomy was monitored for 8 weeks. 
      Serum, lumbosacral (L1-S1) and brain magnesium levels were analyzed 0, 30, 60, 
      120, 180, 240, 360 min and 24 h after the s.c. injection of 600 mg/kg MgSO4. 
      Serum magnesium levels increased quickly from 1.02 mM (0 time) to 4.52 mM (at 60 
      min) and dropped afterwards to reach physiological levels at 6 h. Peak increments 
      in L1-S1 and brain Mg2+ levels were smaller (32% and 30%, respectively) although 
      maintained for at least 6 h. Magnesium pretreatment in a significant and 
      dose-dependent manner (1) largely suppressed autotomy, (2) decreased final 
      autotomy scores, (3) delayed autotomy onset, and (4) decreased the percentage of 
      animals engaged in high autotomy behaviors. The data support a role for 
      excitatory amino acids in determining susceptibility to autotomy and suggest a 
      hopeful way to prevent neuropathic pain in humans after peripheral 
      deafferentation.
FAU - Feria, Manuel
AU  - Feria M
AD  - Departments of Pharmacology, Faculty of Medicine, University of La Laguna, 
      TenerifeSpain Departments of Physiology, Faculty of Medicine, University of La 
      Laguna, TenerifeSpain.
FAU - Abad, Faustino
AU  - Abad F
FAU - Sanchez, Alberto
AU  - Sanchez A
FAU - Abreu, Pedro
AU  - Abreu P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 7487-88-9 (Magnesium Sulfate)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Denervation
MH  - Injections, Subcutaneous
MH  - Magnesium Sulfate/administration & dosage/pharmacokinetics/*pharmacology
MH  - Male
MH  - Neurons, Afferent/*physiology
MH  - Pain/*psychology
MH  - Peripheral Nerves/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/physiology
MH  - Self Mutilation/*psychology
MH  - Spinal Cord/metabolism
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 00006396-199306000-00007 [pii]
AID - 10.1016/0304-3959(93)90225-E [doi]
PST - ppublish
SO  - Pain. 1993 Jun;53(3):287-293. doi: 10.1016/0304-3959(93)90225-E.