PMID- 8353810
OWN - NLM
STAT- MEDLINE
DCOM- 19930921
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 68
IP  - 2
DP  - 1993 Jul 15
TI  - Rationalization of in situ hybridization: testing up to 16 different probes on a 
      single slide.
PG  - 91-4
AB  - Although particularly interested in tumor research, investigators in many tumor 
      cytogenetics laboratories cannot afford extensive molecular/interphase 
      cytogenetics studies in addition to routine cytogenetics analyses, primarily 
      because many slides must be stained to examine a few cases using a panel of 
      centromeric probes. Moreover, fluorescence in situ hybridization (FISH) 
      techniques mostly require freshly prepared reagents, such as hybridization 
      mixtures or antibody solutions. These technical requirements are very 
      time-consuming, thus limiting their use in routine screening. We report a 
      significantly economical method for rapid performance of interphase cytogenetics 
      in great numbers of cases. The major advantage if its superior efficiency: up to 
      16 different probes may be used on one single slide. Moreover, the method is 
      significantly cheaper than other techniques owing to minimal probe consumption. 
      The technique is also best suited if great numbers of new probes, e.g., 
      polymerase chain reaction (PCR)-generated YAC-probes, must be tested for their 
      applicability in FISH.
FAU - Weber-Matthiesen, K
AU  - Weber-Matthiesen K
AD  - Institut fur Humangenetik, Klinikum der Christian-Albrechts-Universitat zu Kiel, 
      Germany.
FAU - Deerberg, J
AU  - Deerberg J
FAU - Muller-Hermelink, A
AU  - Muller-Hermelink A
FAU - Winkemann, M
AU  - Winkemann M
FAU - Schlegelberger, B
AU  - Schlegelberger B
FAU - Grote, W
AU  - Grote W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosomes, Human, Pair 1/*ultrastructure
MH  - *DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/economics/*methods
MH  - Sensitivity and Specificity
EDAT- 1993/07/15 00:00
MHDA- 1993/07/15 00:01
CRDT- 1993/07/15 00:00
PHST- 1993/07/15 00:00 [pubmed]
PHST- 1993/07/15 00:01 [medline]
PHST- 1993/07/15 00:00 [entrez]
AID - 0165-4608(93)90002-4 [pii]
AID - 10.1016/0165-4608(93)90002-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1993 Jul 15;68(2):91-4. doi: 
      10.1016/0165-4608(93)90002-4.