PMID- 8360595
OWN - NLM
STAT- MEDLINE
DCOM- 19930929
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 54
IP  - 2
DP  - 1993 Aug
TI  - Suppression of human IgE antibody forming cell responses by IL-6.
PG  - 168-70
AB  - To study the effects of cytokines on human IgE antibody forming cells (AFCs), log 
      phase U266 myeloma cells (3 x 10(3)/ml), which secrete immunoglobulin E (IgE), 
      were cultured for 0-24 h with and without cytokine or with or without antibodies 
      against various cytokines. The numbers of IgE AFCs were determined in ELISPOT 
      assay. We found that interleukin-6 (IL-6) suppressed (to 95%) whereas anti-IL-6 
      increased (to 148%) the numbers of IgE AFCs and that both worked in a 
      dose-dependent fashion. IL-4 and interferon-gamma (IFN-gamma) also suppressed IgE 
      AFC responses in a dose-dependent fashion. However, antibodies to these cytokines 
      had no effect. In contrast, IFN-alpha increased (to fourfold) the numbers of IgE 
      AFCs in a dose-dependent fashion. The data are the first to show a suppressive 
      effect of IL-6 on human IgE responses and may also suggest a role for IL-6 in the 
      treatment of atopic disease.
FAU - Kleiner, G I
AU  - Kleiner GI
AD  - Department of Pathology State University of New York Health Science Center at 
      Brooklyn 11203.
FAU - Auci, D L
AU  - Auci DL
FAU - Shaikh, A
AU  - Shaikh A
FAU - Chice, S M
AU  - Chice SM
FAU - Durkin, H G
AU  - Durkin HG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-6)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibody-Producing Cells/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immune Tolerance/*physiology
MH  - Immunoglobulin E/*biosynthesis
MH  - Interferon-alpha/physiology
MH  - Interferon-gamma/physiology
MH  - Interleukin-4/physiology
MH  - Interleukin-6/*physiology
MH  - Tumor Cells, Cultured
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - 10.1002/jlb.54.2.168 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1993 Aug;54(2):168-70. doi: 10.1002/jlb.54.2.168.