PMID- 8361501
OWN - NLM
STAT- MEDLINE
DCOM- 19930924
LR  - 20131121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 7
IP  - 6
DP  - 1993 Jun
TI  - An element of the transforming growth factor-beta 1 5'-untranslated region 
      represses translation and specifically binds a cytosolic factor.
PG  - 759-66
AB  - In many cell types, there is a discrepancy between transforming growth 
      factor-beta 1 (TGF-beta 1) mRNA and TGF-beta 1 protein, suggesting that 
      expression of TGF-beta 1 is regulated posttranscriptionally. We have previously 
      shown that a 137-nucleotide (nt) region of the TGF-beta 1 5'-untranslated region 
      (UTR) potently inhibits the expression of a heterologous reporter gene, 
      suggesting a role for this region in the posttranscriptional inhibition of 
      TGF-beta 1 expression. To study the mechanism of inhibition, a chimeric plasmid 
      containing this region of the TGF-beta 1 5'-UTR and the reading frame of the 
      human GH gene was stably transfected into C2C12 myoblastic cells. Our results 
      show that the TGF-beta 1 5'-UTR inhibits GH expression by inhibiting GH mRNA 
      translation. In vitro gel retardation and cross-linking assays using a 
      radiolabelled RNA probe transcribed from this region of the TGF-beta 1 5'-UTR 
      demonstrate the specific binding of a cytosolic factor. Deletion of a potential 
      stem-loop-forming region abolishes binding of this factor and partially restores 
      GH production. These results suggest that posttranscriptional inhibition of 
      TGF-beta 1 expression is at the level of mRNA translation and that a cytosolic 
      factor may regulate TGF-beta 1 mRNA translation.
FAU - Romeo, D S
AU  - Romeo DS
AD  - National Cancer Institute, Laboratory of Chemoprevention, Bethesda, Maryland 
      20892.
FAU - Park, K
AU  - Park K
FAU - Roberts, A B
AU  - Roberts AB
FAU - Sporn, M B
AU  - Sporn MB
FAU - Kim, S J
AU  - Kim SJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 9002-72-6 (Growth Hormone)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cytosol/*metabolism
MH  - Dactinomycin/pharmacology
MH  - Gene Expression
MH  - Growth Hormone/genetics
MH  - Humans
MH  - Mice
MH  - Muscles/metabolism
MH  - Nucleic Acid Hybridization
MH  - Protein Binding
MH  - Protein Biosynthesis/*drug effects
MH  - RNA Probes
MH  - RNA, Messenger/genetics/metabolism/*pharmacology
MH  - Ribonucleases/metabolism
MH  - Transfection
MH  - Transforming Growth Factor beta/*genetics
MH  - Ultraviolet Rays
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 10.1210/mend.7.6.8361501 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1993 Jun;7(6):759-66. doi: 10.1210/mend.7.6.8361501.