PMID- 83648
OWN - NLM
STAT- MEDLINE
DCOM- 19790313
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 75
IP  - 12
DP  - 1978 Dec
TI  - Differential expression of poly(A)-adjacent sequences of mammary tumor virus RNA 
      in murine mammary cells.
PG  - 5797-801
AB  - Two DNA probes representative of either the entire mouse mammary tumor virus 
      (MMTV) genome or the poly(A)-adjacent sequences at the 3' end of MMTV RNA were 
      synthesized with calf thymus DNA or oligo(dT) primers, respectively. These probes 
      were used to study the expression of endogenous MMTV sequences in several BALB/c 
      mammary tumor cell lines, in normal lactating BALB/c tissue, and in a cloned C3H 
      tumor cell line. Both probes were characterized with respect to their rates of 
      hybridization with template RNA, their size as determined by alkaline sucrose 
      gradient centrifugation, and the thermal stability of the cDNA.MMTV RNA hybrids. 
      In addition, the ability of the calf thymus oligodeoxy-nucleotide- or 
      oligo(dT)-primed probes to protect (125)I-labeled MMTV RNA or (125)I-labeled 
      poly(A)-adjacent MMTV RNA sequences from S1 nuclease digestion was determined. 
      Hybridization analysis with these two probes indicated that (i) there were 
      approximately 20-fold more oligo(dT)-primed sequences in BALB/c lactating tissue 
      than there were sequences representing the entire genome; (ii) in BALB/c tumor 
      cells, the oligo(dT):random oligonucleotide-primed cDNA sequence ratio was 
      reduced to 4:1; and (iii) in virus-producer C3H tumor cells, there was only a 
      2-fold excess of oligo(dT)-primed sequences over that observed with a 
      representative cDNA. These results are consistent with the presence of subgenomic 
      viral mRNA species, integration of partial proviral copies, or altered mRNA 
      processing.
FAU - Dudley, J P
AU  - Dudley JP
FAU - Rosen, J M
AU  - Rosen JM
FAU - Butel, J S
AU  - Butel JS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 24937-83-5 (Poly A)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Female
MH  - *Genes, Viral
MH  - *Lactation
MH  - Mammary Glands, Animal/*microbiology
MH  - Mammary Neoplasms, Experimental/*genetics/microbiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Nucleic Acid Hybridization
MH  - Poly A/genetics
MH  - Pregnancy
MH  - RNA-Directed DNA Polymerase/metabolism
PMC - PMC393062
EDAT- 1978/12/01 00:00
MHDA- 1978/12/01 00:01
PMCR- 1979/06/01
CRDT- 1978/12/01 00:00
PHST- 1978/12/01 00:00 [pubmed]
PHST- 1978/12/01 00:01 [medline]
PHST- 1978/12/01 00:00 [entrez]
PHST- 1979/06/01 00:00 [pmc-release]
AID - 10.1073/pnas.75.12.5797 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1978 Dec;75(12):5797-801. doi: 10.1073/pnas.75.12.5797.