PMID- 8365564
OWN - NLM
STAT- MEDLINE
DCOM- 19931006
LR  - 20061115
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 159
IP  - 1
DP  - 1993 Sep
TI  - Brain-derived neurotrophic factor and neurotrophin-3 induce cell proliferation in 
      the cochleovestibular ganglion through a glycosyl-phosphatidylinositol signaling 
      system.
PG  - 257-65
AB  - We have investigated the role of brain-derived neurotrophic factor (BDNF) and 
      neurotrophin-3 (NT-3) in the regulation of cell proliferation in the early 
      developing cochleovestibular ganglion (CVG). Ganglia were isolated from 72-hr 
      chick embryos and cultured for 24 hr. Both BDNF and NT-3 had a powerful mitogenic 
      effect, at doses of 1-5 ng/ml, consistent with an involvement of the 
      high-affinity receptor. Evidence for the participation of the 
      glycosyl-phosphatidylinositol (GPI)/inositol phosphoglycan (IPG) signaling system 
      in the mediation of proliferative effects of BDNF and NT-3 is presented. Both of 
      these neurotrophins elicited a fast and transient hydrolysis of labeled GPI, 
      approximately 60% in 30 sec. The dose-response profile of GPI hydrolysis overlaps 
      the neurotrophin-induced cell proliferation response profile. Anti-IPG antibodies 
      were able to block the growth-promoting effects of BDNF and NT-3. Anti-IPG 
      antibodies immunoprecipitated a CVG-endogenous IPG, induced upon BDNF treatment, 
      which exhibited proliferative stimulating properties. Both BDNF and NT-3 are 
      proposed as potential candidates for regulation of growth during CVG development, 
      with this mitogenic effect being mediated by the GPI/IPG signaling system.
FAU - Represa, J
AU  - Represa J
AD  - Departamento Bioquimica, Biologia Molecular y Fisiologia, Facultad de Medicina, 
      Universidad de Valladolid, Spain.
FAU - Avila, M A
AU  - Avila MA
FAU - Romero, G
AU  - Romero G
FAU - Mato, J M
AU  - Mato JM
FAU - Giraldez, F
AU  - Giraldez F
FAU - Varela-Nieto, I
AU  - Varela-Nieto I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Inositol Phosphates)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Polysaccharides)
RN  - 0 (inositol phosphate glycan)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Division
MH  - Chick Embryo
MH  - Cochlear Nerve/cytology/*metabolism
MH  - Culture Techniques
MH  - Ganglia/cytology/metabolism
MH  - Glycosylphosphatidylinositols/immunology/*metabolism
MH  - Inositol Phosphates/immunology
MH  - Kinetics
MH  - Nerve Growth Factors/*physiology
MH  - Nerve Tissue Proteins/*physiology
MH  - Neurotrophin 3
MH  - Polysaccharides/immunology
MH  - *Signal Transduction
MH  - Vestibular Nerve/cytology/*metabolism
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - S0012-1606(83)71238-8 [pii]
AID - 10.1006/dbio.1993.1238 [doi]
PST - ppublish
SO  - Dev Biol. 1993 Sep;159(1):257-65. doi: 10.1006/dbio.1993.1238.