PMID- 8365565 OWN - NLM STAT- MEDLINE DCOM- 19931006 LR - 20071114 IS - 0012-1606 (Print) IS - 0012-1606 (Linking) VI - 159 IP - 1 DP - 1993 Sep TI - Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons. PG - 266-75 AB - The effects of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) on the differentiation of avian cochleovestibular ganglion and their possible association with the hydrolysis of glycosyl-phosphatidylinositol (GPI) were studied. BDNF and NT-3 (2 ng/ml) promoted neurite outgrowth in explants of both cochlear and vestibular ganglia. This effect on neuritogenesis was stage-dependent, reaching a maximum at E7 for NT-3 and at E9 for BDNF. The magnitude of the response of the vestibular ganglion to BDNF was always smaller than that of the cochlear ganglion of an equivalent stage. BDNF and NT-3 stimulation of neuronal survival and neurite extension was also demonstrated in dissociated neuronal cell cultures. The effect was concentration-dependent with saturation of the response occurring at 4 ng/ml for BDNF and at 2 ng/ml for NT-3, the half-maximal effect occurring at 2 and 1 ng/ml, respectively, for the most sensitive stages of the chick cochlear ganglion. Inositol phosphoglycan (IPG) did not mimic the effects of BDNF or NT-3 on neuronal survival and neurite outgrowth, nor was it able to potentiate their responses. Antibodies raised against IPG did not block the effects of these neurotrophins. The results suggest that BDNF and NT-3 may act in cooperation to establish the innervation pattern of the inner ear. Unlike their early proliferative effects, neurotrophic effects are uncoupled from the GPI/IPG signal transduction system. FAU - Avila, M A AU - Avila MA AD - Instituto de Investigaciones Biomedicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain. FAU - Varela-Nieto, I AU - Varela-Nieto I FAU - Romero, G AU - Romero G FAU - Mato, J M AU - Mato JM FAU - Giraldez, F AU - Giraldez F FAU - Van De Water, T R AU - Van De Water TR FAU - Represa, J AU - Represa J LA - eng GR - DC00088/DC/NIDCD NIH HHS/United States GR - NIDDK 40753/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Dev Biol JT - Developmental biology JID - 0372762 RN - 0 (Antibodies) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glycosylphosphatidylinositols) RN - 0 (Inositol Phosphates) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Neurotrophin 3) RN - 0 (Polysaccharides) RN - 0 (inositol phosphate glycan) SB - IM MH - Animals MH - Antibodies/immunology MH - Brain-Derived Neurotrophic Factor MH - Cell Differentiation MH - Cell Survival MH - Chick Embryo MH - Cochlear Nerve/*cytology/growth & development MH - Culture Techniques MH - Ear, Inner/growth & development/innervation MH - Ganglia/cytology MH - Glycosylphosphatidylinositols/metabolism MH - Inositol Phosphates/immunology/metabolism MH - Nerve Growth Factors/*physiology MH - Nerve Tissue Proteins/*physiology MH - *Neurites MH - Neurons/cytology MH - Neurotrophin 3 MH - Polysaccharides/immunology/metabolism MH - Signal Transduction MH - Vestibular Nerve/*cytology/growth & development EDAT- 1993/09/01 00:00 MHDA- 1993/09/01 00:01 CRDT- 1993/09/01 00:00 PHST- 1993/09/01 00:00 [pubmed] PHST- 1993/09/01 00:01 [medline] PHST- 1993/09/01 00:00 [entrez] AID - S0012-1606(83)71239-X [pii] AID - 10.1006/dbio.1993.1239 [doi] PST - ppublish SO - Dev Biol. 1993 Sep;159(1):266-75. doi: 10.1006/dbio.1993.1239.