PMID- 8369082
OWN - NLM
STAT- MEDLINE
DCOM- 19931014
LR  - 20061115
IS  - 0935-8943 (Print)
IS  - 0935-8943 (Linking)
VI  - 72
IP  - 7
DP  - 1993 Jul
TI  - [Autocrine growth mechanisms of cholesteatoma epithelium].
PG  - 319-23
AB  - Transforming growth factor alpha (TGF alpha) and interleukin 1 alpha (IL-1 alpha) 
      are known to be produced by normal human keratinocytes stimulating their 
      proliferation. The distribution and expression of TGF alpha and IL-1 alpha were 
      examined in specimens of middle ear cholesteatoma by means of immunohistochemical 
      methods using a monoclonal antibody against TGF alpha and a polyclonal one 
      against IL-1 alpha. Normal retroauricular skin was stained for comparison. 
      Staining for TGF alpha was consistently stronger in cholesteatoma epithelium than 
      in normal epidermis, and encompassed all epithelial cell layers. Immune cells 
      occurring in the stroma of cholesteatoma also reacted positively for TGF alpha. 
      The intensity of staining for IL-1 alpha was markedly stronger in cholesteatoma 
      tissue than in normal epidermis. All cellular layers of the squamous epithelium 
      of cholesteatoma stained strongly and uniformly for IL-1 alpha, whereas the 
      keratin layer was negative for IL-1 alpha. In the connective tissue beneath the 
      cholesteatoma epithelium intensely positive cells were scattered between negative 
      stromal cells. These data are consistent with autocrine stimulation of the 
      squamous epithelium of cholesteatoma by TGF alpha and IL-1 alpha as well as with 
      a paracrine stimulation by immune cells. Both factors contribute to the 
      unrestrained growth of cholesteatoma in the middle ear cavity.
FAU - Schilling, V
AU  - Schilling V
AD  - Klinikum Grosshadern, Ludwig-Maximilians-Universitat Munchen.
FAU - Holly, A
AU  - Holly A
FAU - Bujia, J
AU  - Bujia J
FAU - Schulz, P
AU  - Schulz P
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Autokrine Wachstumsmechanismen des Cholesteatomepithels.
PL  - Germany
TA  - Laryngorhinootologie
JT  - Laryngo- rhino- otologie
JID - 8912371
RN  - 0 (Interleukin-1)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Cell Division/*physiology
MH  - Cholesteatoma/*pathology/surgery
MH  - Ear Diseases/*pathology/surgery
MH  - Ear, Middle/*pathology/surgery
MH  - Epithelium/pathology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-1/*physiology
MH  - Transforming Growth Factor alpha/*physiology
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1055/s-2007-997908 [doi]
PST - ppublish
SO  - Laryngorhinootologie. 1993 Jul;72(7):319-23. doi: 10.1055/s-2007-997908.