PMID- 8370520
OWN - NLM
STAT- MEDLINE
DCOM- 19931013
LR  - 20190523
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 7
IP  - 9
DP  - 1993 Sep
TI  - A bZIP protein, sisterless-a, collaborates with bHLH transcription factors early 
      in Drosophila development to determine sex.
PG  - 1688-702
AB  - Sexual identity in Drosophila is determined by zygotic X-chromosome dose. Two 
      potent indicators of X-chromosome dose are sisterless-a (sis-a) and sisterless-b 
      (sis-b). Genetic analysis has shown that a diplo-X dose of these genes activates 
      their regulatory target, the feminizing switch gene Sex-lethal (Sxl), whereas a 
      haplo-X dose leaves Sxl inactive. sis-b encodes a transcriptional activator of 
      the bHLH family that dimerizes with several other HLH proteins required for the 
      proper assessment of X dose. Here, we report that sis-a encodes a bZIP protein 
      homolog that functions in all somatic nuclei to activate Sxl transcription. In 
      contrast with other elements of the sex-determination signal, the functioning of 
      this transcription factor in somatic cells may be specific to X-chromosome 
      counting. Using in situ hybridization, we determined the time course of sis-a, 
      sis-b, and Sxl transcription during the first few hours after fertilization. The 
      pattern of sis-a RNA accumulation is very similar to that for sis-b, with a peak 
      in nuclear cycle 12 at about the time of onset of Sxl transcription. Considered 
      in the context of other studies, these results suggest that the ability to 
      distinguish one X from two is attributable to combinatorial interactions between 
      bZIP and bHLH proteins and their target, Sxl, as well as to positive and negative 
      interactions with maternally supplied and zygotically produced proteins.
FAU - Erickson, J W
AU  - Erickson JW
AD  - Department of Molecular and Cell Biology, University of California, Berkeley 
      94720.
FAU - Cline, T W
AU  - Cline TW
LA  - eng
SI  - GENBANK/L11593
SI  - GENBANK/L11594
SI  - GENBANK/L16016
SI  - GENBANK/L21156
SI  - GENBANK/L22755
SI  - GENBANK/L24529
SI  - GENBANK/S56762
SI  - GENBANK/S56763
SI  - GENBANK/Z15047
SI  - GENBANK/Z15048
GR  - GM23468/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Hormones)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Sxl protein, Drosophila)
RN  - 0 (Transcription Factors)
RN  - 0 (sisA protein, Drosophila)
SB  - IM
GS  - Sxl
GS  - sis-a
GS  - sis-b
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basic-Leucine Zipper Transcription Factors
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Drosophila/*embryology
MH  - *Drosophila Proteins
MH  - Female
MH  - Genes, Lethal
MH  - Insect Hormones/genetics
MH  - *Leucine Zippers
MH  - Male
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - RNA, Messenger/metabolism
MH  - *RNA-Binding Proteins
MH  - Sequence Homology, Amino Acid
MH  - Sex Differentiation/*genetics
MH  - Transcription Factors/genetics/*metabolism
MH  - Transformation, Genetic
MH  - X Chromosome
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1101/gad.7.9.1688 [doi]
PST - ppublish
SO  - Genes Dev. 1993 Sep;7(9):1688-702. doi: 10.1101/gad.7.9.1688.