PMID- 8370705
OWN - NLM
STAT- MEDLINE
DCOM- 19931014
LR  - 20131121
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 77
IP  - 3
DP  - 1993 Sep
TI  - In vitro production of cytokines by bone surface-derived osteoblastic cells in 
      normal and osteoporotic postmenopausal women: relationship with cell 
      proliferation.
PG  - 824-30
AB  - To evaluate the role of cytokines produced by osteoblasts in the pathophysiology 
      of bone lesions in postmenopausal osteoporosis (PMOP), we have determined by RIA 
      and immunoradiometric assays the levels of prostaglandin E2 (PGE2), interleukin-1 
      beta (IL-1), tumor necrosis factor-alpha (TNF alpha), and IL-6 released by 
      cultured bone surface-derived osteoblastic (OB) cells isolated from 24 untreated 
      PMOP women with high, low, or normal bone turnover on bone biopsy. OB cells 
      isolated from patients with high bone formation had a 2-fold increased 
      proliferation rate in vitro compared to OB cells from patients with normal or low 
      bone formation or OB cells from age-matched controls. The spontaneous in vitro 
      production per cell protein of PGE2, IL-1, and TNF alpha, but not of IL-6, was 2- 
      to 3-fold lower in rapidly proliferating OB cells isolated from PMOP patients 
      with high bone formation compared to OB cells from patients with normal or low 
      proliferation or control cells. Treatment with 10 nmol/L 1,25-dihydroxyvitamin D 
      (48 h) increased PGE2 levels to normal values in OB cells with a high 
      proliferation rate, but decreased PGE2 production in cells with low proliferation 
      and in control cells, suggesting that the release of PGE2 was dependent on the 
      stage of maturation of OB cells. Significant correlations were found between IL-1 
      and TNF alpha (r = 0.87; P < 0.001), IL-1 and PGE2 (r = 0.46; P < 0.05), IL-6 and 
      IL-1 (r = 0.39; P < 0.05), and IL-6 and TNF alpha (r = 0.49; P < 0.05), 
      suggesting that the production of these cytokines was under reciprocal control. 
      The results indicate that the in vitro production of PGE2, IL-1, and TNF alpha, 
      but not IL-6, by OB cells isolated from patients with PMOP is related to the 
      proliferation rate of these cells and the rate of bone formation. The variable 
      production of cytokines by OB cells may contribute to the histological 
      heterogeneity of bone formation in PMOP.
FAU - Marie, P J
AU  - Marie PJ
AD  - Unite 349 INSERM (Cell and Molecular Biology of Bone and Cartilage), Lariboisiere 
      Hospital, Paris, France.
FAU - Hott, M
AU  - Hott M
FAU - Launay, J M
AU  - Launay JM
FAU - Graulet, A M
AU  - Graulet AM
FAU - Gueris, J
AU  - Gueris J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
RN  - FXC9231JVH (Calcitriol)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Calcitriol/pharmacology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis
MH  - DNA/biosynthesis
MH  - Dinoprostone/biosynthesis
MH  - Female
MH  - Humans
MH  - Interleukin-1/biosynthesis
MH  - Middle Aged
MH  - Osteoblasts/drug effects/*metabolism/pathology
MH  - Osteoporosis, Postmenopausal/*metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1210/jcem.77.3.8370705 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1993 Sep;77(3):824-30. doi: 10.1210/jcem.77.3.8370705.