PMID- 8371350
OWN - NLM
STAT- MEDLINE
DCOM- 19931012
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 67
IP  - 10
DP  - 1993 Oct
TI  - Dengue virus-specific human CD4+ T-lymphocyte responses in a recipient of an 
      experimental live-attenuated dengue virus type 1 vaccine: bulk culture 
      proliferation, clonal analysis, and precursor frequency determination.
PG  - 5962-7
AB  - We analyzed the CD4+ T-lymphocyte responses to dengue, West Nile, and yellow 
      fever viruses 4 months after immunization of a volunteer with an experimental 
      live-attenuated dengue virus type 1 vaccine (DEN-1 45AZ5). We examined bulk 
      culture proliferation to noninfectious antigens, determined the precursor 
      frequency of specific CD4+ T cells by limiting dilution, and established and 
      analyzed CD4+ T-cell clones. Bulk culture proliferation was predominantly dengue 
      virus type 1 specific with a lesser degree of cross-reactive responses to other 
      dengue virus serotypes, West Nile virus, and yellow fever virus. Precursor 
      frequency determination by limiting dilution in the presence of noninfectious 
      dengue virus antigens revealed a frequency of antigen-reactive cells of 1 in 
      1,686 peripheral blood mononuclear cells (PBMC) for dengue virus type 1, 1 in 
      9,870 PBMC for dengue virus type 3, 1 in 14,053 PBMC for dengue virus type 2, and 
      1 in 17,690 PBMC for dengue virus type 4. Seventeen CD4+ T-cell clones were then 
      established by using infectious dengue virus type 1 as antigen. Two patterns of 
      dengue virus specificity were found in these clones. Thirteen clones were dengue 
      virus type 1 specific, and four clones recognized both dengue virus types 1 and 
      3. Analysis of human leukocyte antigen (HLA) restriction revealed that five 
      clones are HLA-DRw52 restricted, one clone is HLA-DP3 restricted, and one clone 
      is HLA-DP4 restricted. These results indicate that in this individual, the CD4+ 
      T-lymphocyte responses to immunization with live-attenuated dengue virus type 1 
      vaccine are predominantly serotype specific and suggest that a multivalent 
      vaccine may be necessary to elicit strong serotype-cross-reactive CD4+ 
      T-lymphocyte responses in such individuals.
FAU - Green, S
AU  - Green S
AD  - Department of Medicine, University of Massachusetts Medical Center, Worcester 
      01655.
FAU - Kurane, I
AU  - Kurane I
FAU - Edelman, R
AU  - Edelman R
FAU - Tacket, C O
AU  - Tacket CO
FAU - Eckels, K H
AU  - Eckels KH
FAU - Vaughn, D W
AU  - Vaughn DW
FAU - Hoke, C H Jr
AU  - Hoke CH Jr
FAU - Ennis, F A
AU  - Ennis FA
LA  - eng
GR  - R01-AI30624/AI/NIAID NIH HHS/United States
GR  - T32-A107272/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Viral)
RN  - 0 (CD3 Complex)
RN  - 0 (CD4 Antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/*analysis
MH  - Antigens, Viral/immunology
MH  - CD3 Complex/analysis
MH  - CD4 Antigens/*analysis
MH  - Cells, Cultured
MH  - Clone Cells
MH  - *Cytotoxicity, Immunologic
MH  - Dengue Virus/classification/*immunology
MH  - HLA-D Antigens/immunology
MH  - Humans
MH  - *Lymphocyte Activation
MH  - Lymphocytes/*microbiology
MH  - Male
MH  - Serotyping
MH  - T-Lymphocyte Subsets/*immunology
MH  - Vaccines, Attenuated/*immunology
MH  - Viral Vaccines/*immunology
PMC - PMC238017
EDAT- 1993/10/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1993/10/01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
PHST- 1993/10/01 00:00 [pmc-release]
AID - 10.1128/JVI.67.10.5962-5967.1993 [doi]
PST - ppublish
SO  - J Virol. 1993 Oct;67(10):5962-7. doi: 10.1128/JVI.67.10.5962-5967.1993.