PMID- 8374611
OWN - NLM
STAT- MEDLINE
DCOM- 19931021
LR  - 20191101
IS  - 1064-1963 (Print)
IS  - 1064-1963 (Linking)
VI  - 15
IP  - 4
DP  - 1993 Jul
TI  - Angiotensinogen mRNA and pressor reactions to angiotensin in brain stem areas of 
      spontaneously hypertensive rats.
PG  - 709-25
AB  - The role of angiotensin (ANG II) at the tissue level, particularly in the brain, 
      remains imperfectly defined. We measured angiotensinogen (A degrees) mRNA in the 
      brain stems, sensory and sympathetic ganglia, and blood vessels of Wistar-Kyoto 
      (WKY) and stroke-prone spontaneously hypertensive rats (SHR-SP) by quantitative, 
      liquid hybridization. We micro-injected ANG II and glutamate into the brain stems 
      of these rats to gain insight into the functional significance of our findings. 
      A. mRNA was found in the dorsolateral, dorsomedial, and ventrolateral pons, as 
      well as in the dorsolateral, dorsomedial, and ventrolateral medulla of both 
      strains. A degrees mRNA was 8-10 pg/micrograms total mRNA higher (p < 0.05) in 
      the dorsomedial medulla (nucleus tractus solitarii) in WKY and SHR-SP (28.27 +/- 
      1.26 and 33.50 +/- 1.42 pg/micrograms RNA respectively) than in the other areas. 
      SHR-SP had higher values (27.22 +/- 1.77 vs. 21.53 +/- 0.57 pg/micrograms mRNA) 
      than WKY (p < 0.05) in the dorsolateral pons (locus coeruleus). A. mRNA was also 
      identified in the optic nerves and chiasm, trigeminal and coeliac ganglia, 
      arteries and veins. Injections of glutamate and ANG II into the dorsomedial, 
      dorsolateral, and ventrolateral medulla increased blood pressure, while ANG II in 
      the dorsal medial pons did not. We conclude that A degrees mRNA is produced to 
      different degrees in brain stem areas which participate in blood pressure 
      regulation. Medullary structures show more response to local ANG II than pontine 
      structures. A degrees mRNA is located in sensory neural tissues as well as 
      sympathetic ganglia. A degrees mRNA is present in both arteries and veins. These 
      findings underscore the scope and complexity of ANG production in tissues.
FAU - Chai, C Y
AU  - Chai CY
AD  - Academia Sinica Department of Pharmacology, National Defense Medical Center, 
      Taipei, Republic of China.
FAU - Hellmann, W
AU  - Hellmann W
FAU - Tseng, C J
AU  - Tseng CJ
FAU - Luft, F C
AU  - Luft FC
FAU - Ganten, D
AU  - Ganten D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Hypertens
JT  - Clinical and experimental hypertension (New York, N.Y. : 1993)
JID - 9305929
RN  - 0 (RNA, Messenger)
RN  - 11002-13-4 (Angiotensinogen)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/pharmacology
MH  - Angiotensinogen/*genetics
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Blood Vessels/metabolism
MH  - Brain Stem/drug effects/*metabolism
MH  - Female
MH  - Male
MH  - Peripheral Nerves/metabolism
MH  - RNA, Messenger/*genetics/metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Tissue Distribution
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.3109/10641969309041638 [doi]
PST - ppublish
SO  - Clin Exp Hypertens. 1993 Jul;15(4):709-25. doi: 10.3109/10641969309041638.