PMID- 8374742
OWN - NLM
STAT- MEDLINE
DCOM- 19931015
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 617
IP  - 1
DP  - 1993 Jul 16
TI  - Identification of acetylcholine receptors in adult rat trigeminal ganglion 
      neurons.
PG  - 37-42
AB  - Nicotinic acetylcholine receptors (nAChRs) were identified in a subpopulation of 
      cultured adult rat trigeminal ganglia (TG) neurons by whole-cell patch-clamp 
      recordings. Dimethylphenylpiperazinium (DMPP), a nAChR agonist, induced inward 
      currents in 21/68 of TG neurons having soma diameters greater than 28 microns. 
      These currents were inhibited by hexamethonium, mecamylamine and atropine, 
      indicating the presence of neuronal ganglionic-type nAChRs. This interpretation 
      is consistent with the finding that the nicotine- or DMPP-induced currents were 
      not inhibited by alpha-bungarotoxin (alpha-Bng) in 5 of the 9 cells tested with 
      this compound. However, in 2 of the 9 cells tested, the DMPP-induced currents 
      were completely inhibited by alpha-Bng, and in the remaining two cells tested, 
      the currents were partially inhibited by alpha-Bng. About 22% of the cells having 
      diameters > or = 28 microns were specifically labeled with FITC-labeled 
      alpha-Bng, whereas only 2% of the cells with soma diameters < 28 microns were 
      labeled. These data taken together suggest that more than one subtype of nAChR is 
      present in TG.
FAU - Liu, L
AU  - Liu L
AD  - Department of Neurobiology, Duke University Medical Center, Durham, NC 27710.
FAU - Pugh, W
AU  - Pugh W
FAU - Ma, H
AU  - Ma H
FAU - Simon, S A
AU  - Simon SA
LA  - eng
GR  - NS 20669/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Receptors, Cholinergic)
RN  - 54-77-3 (Dimethylphenylpiperazinium Iodide)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/*pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Dimethylphenylpiperazinium Iodide/pharmacology
MH  - Female
MH  - Ganglia, Spinal/*drug effects
MH  - Male
MH  - Membrane Potentials/drug effects
MH  - Nerve Fibers/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cholinergic/*analysis
MH  - Trigeminal Ganglion/*chemistry/cytology/drug effects
EDAT- 1993/07/16 00:00
MHDA- 1993/07/16 00:01
CRDT- 1993/07/16 00:00
PHST- 1993/07/16 00:00 [pubmed]
PHST- 1993/07/16 00:01 [medline]
PHST- 1993/07/16 00:00 [entrez]
AID - 0006-8993(93)90609-Q [pii]
AID - 10.1016/0006-8993(93)90609-q [doi]
PST - ppublish
SO  - Brain Res. 1993 Jul 16;617(1):37-42. doi: 10.1016/0006-8993(93)90609-q.