PMID- 8374904
OWN - NLM
STAT- MEDLINE
DCOM- 19931021
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 69
IP  - 1
DP  - 1993 Aug
TI  - Defining the extent and nature of cytogenetic events in prostatic adenocarcinoma: 
      paraffin FISH vs. metaphase analysis.
PG  - 7-12
AB  - A comparative study of primary prostatic tumors utilizing conventional metaphase 
      analysis of prostate tumor cultures and fluorescence in situ hybridization (FISH) 
      analysis of paraffin-embedded tissue sections revealed significant differences in 
      type and extent of cytogenetic aberrations. Clonal trisomy 7 was identified in 
      two tumors by metaphase analysis of prostate cultures, but not confirmed in 
      either case by FISH analysis. True gain of chromosome 8 was revealed by FISH 
      analysis in malignant epithelium of four tumors but not in adjacent normal or 
      hyperplastic glands. Neither gain nor loss of this chromosome was observed by 
      metaphase analysis in any of the tumors. Significant monosomy and nullisomy of 
      chromosome 10 was identified in one case by FISH, but no cells with gain or loss 
      of chromosome 10 were observed by metaphase analysis. Significant loss of the Y 
      chromosome was revealed in one tumor by FISH, but no cells with -Y were 
      identified by metaphase analysis. Clonal loss of the Y chromosome was identified 
      in two other tumors by metaphase analysis. Paraffin FISH analysis of these tumors 
      revealed overall monosomy in both, although in one tumor there was extensive 
      nodular loss of the Y chromosome. Paraffin FISH analysis permits identification 
      of cytogenetic aberrations in areas identified as carcinoma (CaP), prostatic 
      intraepithelial neoplasia (PIN), and benign prostatic hyperplasia (BPH). This 
      technique appears more informative in defining the true extent and nature of 
      cytogenetic aberrations in prostate cancer than metaphase analysis of prostate 
      tumor cultures.
FAU - Micale, M A
AU  - Micale MA
AD  - Department of Pathology, Wayne State University School of Medicine, Detroit, 
      Michigan.
FAU - Sanford, J S
AU  - Sanford JS
FAU - Powell, I J
AU  - Powell IJ
FAU - Sakr, W A
AU  - Sakr WA
FAU - Wolman, S R
AU  - Wolman SR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 10
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 8
MH  - Cytogenetics/*methods
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Metaphase
MH  - Paraffin
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Y Chromosome
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - 0165-4608(93)90103-S [pii]
AID - 10.1016/0165-4608(93)90103-s [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1993 Aug;69(1):7-12. doi: 10.1016/0165-4608(93)90103-s.