PMID- 8376464
OWN - NLM
STAT- MEDLINE
DCOM- 19931021
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 122
IP  - 6
DP  - 1993 Sep
TI  - Synchrony of cell spreading and contraction force as phagocytes engulf large 
      pathogens.
PG  - 1295-300
AB  - A simple micromechanical method has been used to directly measure the force of 
      contraction in single mammalian phagocytes (blood granulocytes) during engulfment 
      of large yeast pathogens. Both the time course of cell spreading over the yeast 
      particle and increase in cell body contractile force were quantitated at three 
      temperatures in the range of 23-35 degrees C. The surprising feature of the 
      phagocyte response was that engulfment and cell body contraction occurred in a 
      serial sequence: i.e., the phagocyte spread rapidly over the particle at a steady 
      rate with no detectable cell body contraction; when spreading stopped, 
      contraction force in the cell body then rose steadily to a plateau level that 
      remained stationary until the next sequence of spreading and contraction. Both 
      spreading and contraction exhibited abrupt start/stop kinetics. Also impressive, 
      the cell contraction force stimulated by phagocytosis was quite large 
      (approximately 10(-8) N)-two orders of magnitude larger than the force necessary 
      to deform passive phagocytes to the same extent. If distributed uniformly over 
      the cell cross section, the contraction force is equivalent to an average 
      contractile stress of approximately 10(3) N/m2 (0.01 Atm). These physical 
      measurements in situ set critical requirements for the mechanism of force 
      generation in granulocytes, imply that a major increase in network cross-linking 
      accompanies build-up in contractile force and that subsequent network dissolution 
      is necessary for locomotion.
FAU - Evans, E
AU  - Evans E
AD  - Department of Pathology, University of British Columbia, Vancouver, Canada.
FAU - Leung, A
AU  - Leung A
FAU - Zhelev, D
AU  - Zhelev D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 9010-72-4 (Zymosan)
SB  - IM
MH  - Biomechanical Phenomena
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Humans
MH  - Phagocytes/metabolism/*physiology
MH  - *Phagocytosis
MH  - Temperature
MH  - Time Factors
MH  - Zymosan/metabolism
PMC - PMC2119861
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
PMCR- 1994/03/02
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
PHST- 1994/03/02 00:00 [pmc-release]
AID - 93388707 [pii]
AID - 10.1083/jcb.122.6.1295 [doi]
PST - ppublish
SO  - J Cell Biol. 1993 Sep;122(6):1295-300. doi: 10.1083/jcb.122.6.1295.