PMID- 8376920
OWN - NLM
STAT- MEDLINE
DCOM- 19931021
LR  - 20171116
IS  - 0022-0744 (Print)
IS  - 0022-0744 (Linking)
VI  - 42
IP  - 3
DP  - 1993 Jun
TI  - Investigation of latex agglutination with different immunoglobulin classes of 
      antibodies by a scanning electron microscopy.
PG  - 135-40
AB  - The conformation of passive latex agglutinations was investigated by a scanning 
      electron microscopy. The complexes of 2,4-dinitrophenyl group (DNP) and bovine 
      serum albumin (BSA) coated microspheres were incubated with monoclonal antibodies 
      to DNP belonging to different classes of immunoglobulins. Examination with a 
      scanning electron microscope demonstrated that the connectors between 
      microspheres, agglutinated with IgE antibody, tended to take long filamentary 
      forms; whereas the connectors of microspheres, agglutinated with IgG or IgM 
      antibodies, formed shorter filaments. These phenomena were confirmed by the 
      agglutination reactions of tick suspension-coated latex and allergic patients' 
      sera. This morphologic difference is probably attributable to the unusually high 
      elasticity of IgE antibody and is an evidence of the high biologic activity of 
      the IgE antibodies. We assumed the processes of latex agglutination whereby the 
      filamentary substances would develop, expecting that the processes could be 
      models of in vivo and in vitro agglutination of cells.
FAU - Matsuzawa, S
AU  - Matsuzawa S
AD  - Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, 
      Japan.
FAU - Kimura, H
AU  - Kimura H
FAU - Nishiye, H
AU  - Nishiye H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Electron Microsc (Tokyo)
JT  - Journal of electron microscopy
JID - 7611157
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Dinitrophenols)
RN  - 0 (Immunoglobulins)
RN  - 0 (dinitrophenyl-bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cattle
MH  - Dinitrophenols/immunology
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - Immunoglobulins/*classification
MH  - Latex Fixation Tests/*methods
MH  - Mice
MH  - Microscopy, Electron, Scanning/*methods
MH  - Microspheres
MH  - Serum Albumin, Bovine/immunology
MH  - Ticks/immunology
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PST - ppublish
SO  - J Electron Microsc (Tokyo). 1993 Jun;42(3):135-40.