PMID- 8378091
OWN - NLM
STAT- MEDLINE
DCOM- 19931018
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 8
IP  - 10
DP  - 1993 Oct
TI  - Rapid isolation and characterization of amplified DNA by chromosome 
      microdissection: identification of IGF1R amplification in malignant melanoma.
PG  - 2827-31
AB  - We describe a novel strategy characterizing gene amplification in human neoplasia 
      based on targeting double minutes (dmin) and homogeneously staining regions (hsr) 
      for chromosome microdissection. This strategy allows the rapid generation of an 
      amplification unit microclone library and permits the rapid identification of the 
      chromosomal origin of the amplified sequences following fluorescence in situ 
      hybridization (FISH). This strategy has been applied to an hsr-bearing malignant 
      melanoma cell line, HA-A, which was then demonstrated to encode multiple 
      overexpressed copies of the IGF1R gene. This strategy combines all steps for 
      detection, cloning, mapping and isolation of amplified gene(s) into a single 
      process that is readily applicable to any specimen carrying cytologic evidence of 
      gene amplification.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Radiation Oncology, University of Michigan, Ann Arbor 48109-0668.
FAU - Trent, J M
AU  - Trent JM
FAU - Meltzer, P S
AU  - Meltzer PS
LA  - eng
GR  - CA-29476/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Neoplasm)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
GS  - IGF1R
GS  - c-fes
MH  - Base Sequence
MH  - *Chromosomes, Human, Pair 15
MH  - DNA, Neoplasm/*analysis
MH  - Gene Amplification/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Insulin-Like Growth Factor I/analysis
MH  - Melanoma/*genetics
MH  - Molecular Sequence Data
MH  - Receptor, IGF Type 1/*genetics
MH  - Skin Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1993 Oct;8(10):2827-31.