PMID- 8378957
OWN - NLM
STAT- MEDLINE
DCOM- 19931018
LR  - 20190727
IS  - 0039-2499 (Print)
IS  - 0039-2499 (Linking)
VI  - 24
IP  - 10
DP  - 1993 Oct
TI  - Evidence from comparative investigations that impaired platelet activation is not 
      specific for stroke-prone spontaneously hypertensive rats.
PG  - 1528-33
AB  - BACKGROUND AND PURPOSE: Platelet behavior of Sprague Dawley (SD), Wistar (WI), 
      Wistar-Kyoto (WKY), spontaneously hypertensive (SHR), and stroke-prone 
      spontaneously hypertensive rats (SHRSP) was studied in vivo to evaluate the 
      importance of hypertension-related hemostatic disorders. METHODS: The study was 
      based on the model of stimulus-induced pulmonary microembolization of labeled 
      platelets. After injection of 51Cr-labeled homologous platelets into 
      urethane-anesthetized rats, the organ distribution of the platelets was 
      continuously monitored by gamma detectors. Count rates of two detectors--one 
      placed above the animals' thoraxes (C1), the other above their abdomens (C2)-and 
      the ratio of C1:C2 were calculated. The following platelet activators were 
      applied intravenously: adenosine diphosphate (ADP; 50 micrograms/kg), collagen 
      (100 micrograms/kg), and thrombin (50 IU/kg). RESULTS: All three substances 
      caused a reversible pulmonary accumulation of the labeled platelets and hence an 
      increase in C1/C2 (delta C1/C2%). ADP induced a shift of 75% in SD, 52% in WI, 
      32% in WKY, 30% in SHR, and 31% in SHRSP. Thrombin-mediated shift was 79% in SD, 
      64% in WI, 58% in WKY, 48% in SHR, and 54% in SHRSP. Collagen induced a shift of 
      85% in SD, 96% in WI, 84% in WKY, 56% in SHR, and 62% in SHRSP. CONCLUSIONS: 
      Because indistinguishable results were observed in both hypertensive strains, we 
      conclude that impaired platelet aggregation is not specific for SHRSP. Hence, it 
      may not primarily be responsible for the increased occurrence of stroke in these 
      animals.
FAU - Klee, A
AU  - Klee A
AD  - Hoechst AG, Werk Kalle-Albert, Wiesbaden, Germany.
FAU - Vater, S
AU  - Vater S
FAU - Schmid-Schonbein, G W
AU  - Schmid-Schonbein GW
FAU - Seiffge, D
AU  - Seiffge D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stroke
JT  - Stroke
JID - 0235266
RN  - 0 (Chromium Radioisotopes)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Cerebrovascular Disorders/blood/genetics
MH  - Chromium Radioisotopes
MH  - Collagen/pharmacology
MH  - Heart Rate/drug effects
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Male
MH  - *Platelet Activation/drug effects
MH  - Platelet Aggregation/drug effects
MH  - Probability
MH  - Rats
MH  - Rats, Inbred SHR/*blood
MH  - Rats, Inbred WKY/blood
MH  - Rats, Sprague-Dawley/blood
MH  - Rats, Wistar/blood
MH  - Species Specificity
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 10.1161/01.str.24.10.1528 [doi]
PST - ppublish
SO  - Stroke. 1993 Oct;24(10):1528-33. doi: 10.1161/01.str.24.10.1528.