PMID- 8381273
OWN - NLM
STAT- MEDLINE
DCOM- 19930303
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 190
IP  - 2
DP  - 1993 Jan 29
TI  - Urokinase-urokinase receptor interaction: non-mitogenic signal transduction in 
      human epidermal cells.
PG  - 347-52
AB  - We studied non-mitogenic signal transduction in a human cell line of epidermal 
      origin which is induced to chemotaxis following stimulation with human 
      urokinase-type plasminogen activator (u-PA) or with the amino-terminal fragment 
      (ATF) of u-PA A chain, which specifically interacts with the cellular receptor. 
      U-PA and ATF stimulated the formation of diacylglycerol (DAG) independently of 
      inositol lipid and phosphatidylcholine turnover, but concomitantly with de novo 
      synthesis from glucose, thus resembling the DAG neosynthesis activated by 
      insulin. DAG was measured in normal epidermal cells and in cells transfected with 
      the human u-PA receptor (u-PAR) gene and stimulated with u-PA or ATF. Transfected 
      clones showed an increase of cell motility under an ATF gradient in vitro as well 
      as an increase of DAG production. These findings identify a novel mechanism of 
      second messenger formation that conveys chemotactic signals upon stimulation of 
      the u-PAR.
FAU - Del Rosso, M
AU  - Del Rosso M
AD  - Institute of General Pathology, Firenze, Italy.
FAU - Anichini, E
AU  - Anichini E
FAU - Pedersen, N
AU  - Pedersen N
FAU - Blasi, F
AU  - Blasi F
FAU - Fibbi, G
AU  - Fibbi G
FAU - Pucci, M
AU  - Pucci M
FAU - Ruggiero, M
AU  - Ruggiero M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Diglycerides)
RN  - 0 (PLAUR protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - IY9XDZ35W2 (Glucose)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Cell Line
MH  - Chemotaxis
MH  - Diglycerides/biosynthesis
MH  - Epidermis/*physiology
MH  - Glucose/metabolism
MH  - Glycerol/metabolism
MH  - Humans
MH  - Peptide Fragments/pharmacology
MH  - Phosphatidylcholines/metabolism
MH  - Receptors, Cell Surface/genetics/*physiology
MH  - Receptors, Urokinase Plasminogen Activator
MH  - *Signal Transduction
MH  - Transfection
MH  - Urokinase-Type Plasminogen Activator/*pharmacology
EDAT- 1993/01/29 00:00
MHDA- 1993/01/29 00:01
CRDT- 1993/01/29 00:00
PHST- 1993/01/29 00:00 [pubmed]
PHST- 1993/01/29 00:01 [medline]
PHST- 1993/01/29 00:00 [entrez]
AID - S0006291X83710545 [pii]
AID - 10.1006/bbrc.1993.1054 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1993 Jan 29;190(2):347-52. doi: 
      10.1006/bbrc.1993.1054.