PMID- 8382394
OWN - NLM
STAT- MEDLINE
DCOM- 19930323
LR  - 20071114
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 193
IP  - 1
DP  - 1993 Mar
TI  - Phylogenetic and structural analyses of MMTV LTR ORF sequences of exogenous and 
      endogenous origins.
PG  - 171-85
AB  - The long terminal repeat (LTR) of mouse mammary tumor virus (MMTV) harbors an 
      open reading frame (ORF) that encodes a glycoprotein and is present in all 
      exogenous and endogenous MMTV proviruses. The ORF protein has been reported to 
      interact with the immune system of mice to cause deletion of specific V 
      beta-bearing subsets of T cells. Twenty-two MMTV LTR ORF sequences were analyzed. 
      Although highly conserved, the MMTV ORF sequences are not identical, with 
      approximately 35% of the total variation clustered at the carboxy terminus. 
      Statistical analysis revealed the presence of two conserved regions in the 
      protein, one of which contained a transmembrane-like domain (residues 45-63). Two 
      potential nuclear localization signals were recognized. Many ORF sequences shared 
      polymorphisms. To analyze relationships, phylogenetic trees were constructed on 
      the basis of alignments of LTR ORF sequences. A tree generated from the 
      carboxy-terminal 35 residues clustered the sequences into three divergent 
      families. The topology of the tree based on the amino-terminal 288 residues 
      differed significantly, with some MMTV sequences rearranged relative to their 
      carboxy-terminal families. A continuum of exogenous-like to endogenous-like 
      character was suggested by the amino-terminal tree. The discordance between the 
      topologies of the two trees suggests that some type of genetic exchange has 
      occurred in the MMTV LTR gene. Mechanisms and implications of such genetic 
      exchange are discussed.
FAU - Brandt-Carlson, C
AU  - Brandt-Carlson C
AD  - Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
FAU - Butel, J S
AU  - Butel JS
FAU - Wheeler, D
AU  - Wheeler D
LA  - eng
GR  - CA09197/CA/NCI NIH HHS/United States
GR  - CA33369/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Conserved Sequence/*genetics
MH  - Genes, Viral/*genetics
MH  - Mammary Tumor Virus, Mouse/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - Phylogeny
MH  - Repetitive Sequences, Nucleic Acid/*genetics
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - S0042-6822(83)71113-X [pii]
AID - 10.1006/viro.1993.1113 [doi]
PST - ppublish
SO  - Virology. 1993 Mar;193(1):171-85. doi: 10.1006/viro.1993.1113.