PMID- 8385423
OWN - NLM
STAT- MEDLINE
DCOM- 19930506
LR  - 20161123
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 235
IP  - 4
DP  - 1993 Apr
TI  - Stage-dependent changes in spermatogenesis and Sertoli cells in relation to the 
      onset of spermatogenic failure following withdrawal of testosterone.
PG  - 547-59
AB  - Rapid and complete withdrawal of intratesticular testosterone was achieved via 
      the destruction of all Leydig cells with the specific Leydig cell cytotoxin 
      ethane dimethanesulphonate (EDS). Restoration of testosterone levels was 
      accomplished by administration of a single dose (25 mg) of testosterone esters 
      (T) known to reverse the antispermatogenic effects of androgen withdrawal. 
      Quantitation of the degenerating germ cells in cross sections of seminiferous 
      tubules (ST) at stages IV-V, VII, IX, and X-XI of the spermatogenic cycle was 
      used as a sensitive biological index of the effects of testosterone withdrawal 
      and restoration upon the function of the Sertoli cells. Compared to control 
      testicular tissues, the mean numbers of pyknotic germ cells per ST cross section 
      at stages VII, IX and X-XI increased significantly (P < 0.01-0.001) between 4 to 
      8 days post-EDS treatment, but only in stage VII tubules was this trend reversed 
      significantly (P < 0.005) within 2 days by T supplementation. In EDS-treated 
      rats, stages VII, VIII, IX, and X-XI also exhibited significant (P < 0.05-0.001) 
      increases (compared to controls) in the volumetric proportions by which 
      intraepithelial vacuoles appeared within the seminiferous tubules. Again, in 
      EDS+T supplemented rats, the appearance of vacuoles was significantly (P < 0.001) 
      suppressed in stage VII and VIII. In contrast to tubules at stages VII-XI, those 
      at stages IV-V were completely unaffected by testosterone withdrawal or 
      replacement. The results show that at selected time intervals after EDS 
      treatment, testosterone supplementation is capable of preventing/reversing these 
      morphological changes within 2 days in stage VII tubules. It is suggested that 
      the induction and subsequent prevention of seminiferous epithelial damage will 
      serve as an important in vivo and in vitro approach for studies on the 
      androgen-mediated changes in Sertoli cell biology during phases of impairment and 
      recovery of their function. Manipulation of adult Sertoli cell function as 
      provided by our model should permit identification of androgen-regulated gene 
      products together with an understanding of their role(s) in normal and abnormal 
      spermatogenesis.
FAU - Kerr, J B
AU  - Kerr JB
AD  - Department of Anatomy, Monash University, Melbourne, Victoria, Australia.
FAU - Millar, M
AU  - Millar M
FAU - Maddocks, S
AU  - Maddocks S
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 0 (Mesylates)
RN  - 3XMK78S47O (Testosterone)
RN  - EW8V7BJ66Q (ethylene dimethanesulfonate)
SB  - IM
MH  - Animals
MH  - Leydig Cells/drug effects
MH  - Male
MH  - Mesylates/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/drug effects/*ultrastructure
MH  - Sertoli Cells/*cytology/drug effects
MH  - Spermatogenesis/*drug effects
MH  - Spermatozoa/*cytology/drug effects
MH  - Testosterone/administration & dosage/*pharmacology
MH  - Time Factors
MH  - Vacuoles/ultrastructure
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - 10.1002/ar.1092350407 [doi]
PST - ppublish
SO  - Anat Rec. 1993 Apr;235(4):547-59. doi: 10.1002/ar.1092350407.