PMID- 8385651
OWN - NLM
STAT- MEDLINE
DCOM- 19930513
LR  - 20190920
IS  - 0105-6263 (Print)
IS  - 0105-6263 (Linking)
VI  - 16
IP  - 1
DP  - 1993 Feb
TI  - Relative roles of testosterone and the germ cell complement in determining 
      stage-dependent changes in protein secretion by isolated rat seminiferous 
      tubules.
PG  - 71-81
AB  - This study has compared the effect of withdrawal of testosterone (+/- 
      replacement) with that of selective depletion of pachytene spermatocytes (PS) or 
      round/elongating spermatids (RS), or both PS and RS, on the level of overall 
      protein secretion by seminiferous tubules (ST) isolated at particular 
      stage-groups of the spermatogenic cycle. Testosterone withdrawal was induced by 
      destroying the Leydig cells with a single injection of ethane-dimethane 
      sulphonate (EDS), with or without concomitant replacement of testosterone by 
      injection; ST at stages II-V, VI-VIII or IX-XII were then isolated from control 
      and treated rats at 4 days after treatment. Methoxyacetic acid (MAA) was 
      administered, in either one or two doses, to selectively destroy 80-100% of 
      pachytene and later spermatocytes; ST at stages I-V, VI-VIII or IX-XIV were then 
      isolated at specific times after treatment such that ST were depleted selectively 
      of either PS, RS or PS+RS. Isolated ST (5 cm) were then cultured for 22 h at 34 
      degrees C with 35S-methionine and its incorporation into secreted proteins then 
      quantified. Based on the incorporation of 35S-methionine, ST at stages VI-VIII 
      showed a significantly higher level of protein secretion than did ST at earlier 
      or later stages. This difference was abolished following testosterone withdrawal 
      but was maintained by testosterone replacement. The normal increase in protein 
      secretion by ST at stages VI-VIII was also prevented if either PS or RS were 
      depleted, whereas depletion of either PS or RS alone had no significant effect on 
      protein secretion by ST at stages I-V, and only the depletion of RS significantly 
      reduced protein secretion by ST at stages IX-XIV. Depletion of both PS+RS reduced 
      protein secretion significantly by ST at all stages. In contrast to the data for 
      total protein secretion, the levels of sulphated glycoprotein (SGP)-1 and SGP-2 
      secreted by ST at stages VI-VIII showed that these two Sertoli cell proteins were 
      unaffected by germ cell depletion except after co-depletion of PS+RS when 
      secretion of SGP-1 was halved.(ABSTRACT TRUNCATED AT 400 WORDS)
FAU - Sharpe, R M
AU  - Sharpe RM
AD  - MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
FAU - Millar, M
AU  - Millar M
FAU - McKinnell, C
AU  - McKinnell C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
RN  - 0 (Acetates)
RN  - 0 (Clusterin)
RN  - 0 (Glycoproteins)
RN  - 0 (Mesylates)
RN  - 0 (Molecular Chaperones)
RN  - 3XMK78S47O (Testosterone)
RN  - EW8V7BJ66Q (ethylene dimethanesulfonate)
RN  - F11T1H7Q7W (methoxyacetic acid)
SB  - IM
MH  - Acetates
MH  - Animals
MH  - Clusterin
MH  - Culture Techniques
MH  - Glycoproteins/*metabolism
MH  - Male
MH  - Mesylates
MH  - *Molecular Chaperones
MH  - Organ Size
MH  - Rats
MH  - Rats, Wistar
MH  - Seminiferous Tubules/anatomy & histology/*metabolism
MH  - Spermatogenesis/*physiology
MH  - Testis/*cytology
MH  - Testosterone/*physiology
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1111/j.1365-2605.1993.tb01155.x [doi]
PST - ppublish
SO  - Int J Androl. 1993 Feb;16(1):71-81. doi: 10.1111/j.1365-2605.1993.tb01155.x.