PMID- 8385762
OWN - NLM
STAT- MEDLINE
DCOM- 19930513
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 151
IP  - 1
DP  - 1993 Mar 5
TI  - Bovine neurofilament-enriched preparations contain kinase activity similar to 
      casein kinase I--neurofilament phosphorylation by casein kinase I (CKI).
PG  - 89-93
AB  - Neurofilament (NF)-enriched preparations from bovine spinal cord contain 
      regulator-independent kinase activities that phosphorylate NF subunits as well as 
      alpha-casein. CKI-7 (N-2-amino ethyl, 5-chloroisoquinoline, 8-sulfonamide), a 
      specific inhibitor of casein kinase I (CKI), inhibits the phosphorylation of NF 
      subunits in the neurofilament preparation. This inhibition occurs at a 
      concentration range identical to concentrations where CKI-7 inhibits rabbit 
      reticulocyte CKI phosphorylation of alpha-casein. Heparin, a specific inhibitor 
      of casein kinase II (CKII), produced only 20% inhibition of 32P incorporation 
      into NF subunits, and only at concentrations 5 to 10-fold higher than those 
      required to inhibit CKII from reticulocytes. CKI from rabbit reticulocytes 
      phosphorylated all three NF subunits (NF-H, NF-M and NF-L). Comparison of the 
      tryptic phosphopeptide maps of NF-M, phosphorylated by the NF-associated kinase 
      and CKI, indicates that the casein kinase I phosphorylates many of the peptides 
      phosphorylated by the NF-associated kinase and this phosphorylation occurs at the 
      carboxy terminal tail domain of the NF-M subunit. These studies suggest that the 
      major independent kinase activity associated with NFs is CKI.
FAU - Link, W T
AU  - Link WT
AD  - Laboratory of Neurochemistry, NINDS, National Institutes of Health, Bethesda, MD 
      20892.
FAU - Dosemeci, A
AU  - Dosemeci A
FAU - Floyd, C C
AU  - Floyd CC
FAU - Pant, H C
AU  - Pant HC
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Isoquinolines)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 120615-25-0 (N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide)
RN  - 9005-49-6 (Heparin)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Casein Kinases)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoradiography
MH  - Casein Kinases
MH  - Cattle
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Heparin/pharmacology
MH  - Hydrolysis
MH  - In Vitro Techniques
MH  - Intermediate Filaments/*enzymology
MH  - Isoquinolines/pharmacology
MH  - Molecular Sequence Data
MH  - Neurofilament Proteins/*metabolism
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - Phosphotransferases/*metabolism
MH  - Protein Kinase Inhibitors
MH  - Protein Kinases/*metabolism
MH  - Rabbits
MH  - Reticulocytes/drug effects/enzymology
MH  - Spinal Cord/cytology/metabolism
MH  - Trypsin
EDAT- 1993/03/05 00:00
MHDA- 1993/03/05 00:01
CRDT- 1993/03/05 00:00
PHST- 1993/03/05 00:00 [pubmed]
PHST- 1993/03/05 00:01 [medline]
PHST- 1993/03/05 00:00 [entrez]
AID - 0304-3940(93)90053-N [pii]
AID - 10.1016/0304-3940(93)90053-n [doi]
PST - ppublish
SO  - Neurosci Lett. 1993 Mar 5;151(1):89-93. doi: 10.1016/0304-3940(93)90053-n.