PMID- 8386870
OWN - NLM
STAT- MEDLINE
DCOM- 19930527
LR  - 20081121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 194
IP  - 1
DP  - 1993 May
TI  - Activation of both Wnt-1 and Fgf-3 by insertion of mouse mammary tumor virus 
      downstream in the reverse orientation: a reappraisal of the enhancer insertion 
      model.
PG  - 157-65
AB  - In retrovirus-induced tumors, proviral DNA is commonly found next to or within a 
      cellular proto-oncogene such that transcription of the gene is influenced by the 
      viral promoter or enhancer. Extensive surveys of naturally occurring tumors 
      reveal that proviruses integrated on the 3' side of the gene are usually in the 
      same transcriptional orientation, suggesting a model in which the bidirectional 
      viral enhancer acts primarily on the closest promoters. Here we describe a 
      virally induced mammary tumor that appears to contradict these ideas since the 
      Wnt-1/int-1 and Fgf-3/int-2 proto-oncogenes have both been activated by mouse 
      mammary tumor virus (MMTV) DNA integrated 3' of the gene in the opposite 
      transcriptional orientation. However, by cloning the relevant DNAs, we show that 
      these are not simple proviral insertions. In the Wnt-1 locus, there is an 
      additional LTR immediately adjacent to the 3' end of the MMTV provirus, while in 
      Fgf-3, the provirus has sustained a deletion that removes the 5' LTR, gag, and 
      most of pol. These structural alterations can be reconciled with the enhancer 
      insertion model by postulating that the viral enhancer can only function if it is 
      not transcribed.
FAU - Clausse, N
AU  - Clausse N
AD  - Imperial Cancer Research Fund Laboratories, London, United Kingdom.
FAU - Baines, D
AU  - Baines D
FAU - Moore, R
AU  - Moore R
FAU - Brookes, S
AU  - Brookes S
FAU - Dickson, C
AU  - Dickson C
FAU - Peters, G
AU  - Peters G
LA  - eng
SI  - GENBANK/X68450
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (Fgf3 protein, mouse)
RN  - 0 (Fibroblast Growth Factor 3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt1 Protein)
RN  - 0 (Wnt1 protein, mouse)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (fgf3 protein, zebrafish)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
GS  - Fgf-3
GS  - Wnt-1
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Viral/analysis/genetics
MH  - Enhancer Elements, Genetic/*genetics
MH  - Evaluation Studies as Topic
MH  - Fibroblast Growth Factor 3
MH  - *Fibroblast Growth Factors
MH  - Genes, gag/genetics
MH  - Genes, pol/genetics
MH  - Genome, Viral
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proviruses/*genetics
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/analysis
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Sequence Analysis, DNA
MH  - *Transcription, Genetic
MH  - Virus Integration/genetics
MH  - Wnt Proteins
MH  - Wnt1 Protein
MH  - *Zebrafish Proteins
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - S0042-6822(83)71245-6 [pii]
AID - 10.1006/viro.1993.1245 [doi]
PST - ppublish
SO  - Virology. 1993 May;194(1):157-65. doi: 10.1006/viro.1993.1245.